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UBC9 Monoclonal antibody, PBS Only

UBC9 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4A10B11

Cat no : 60201-1-PBS

Print datasheet

Synonyms

C358B7.1, E2,UBE2I, P18, SUMO conjugating enzyme UBC9, SUMO protein ligase, UBC9, UBCE9, UBE2I, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin protein ligase I

验证数据展示

  • WB analysis using 60201-1-Ig (same clone as 60201-1-PBS)

    WB analysis using 60201-1-Ig (same clone as 60201-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60201-1-Ig (UBC9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60201-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 60201-1-Ig (same clone as 60201-1-PBS)

    IHC staining of human lymphoma using 60201-1-Ig (same clone as 60201-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60201-1-Ig (UBC9 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60201-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBE2I/UBC9 antibody included
  • IHC staining of human lymphoma using 60201-1-Ig (same clone as 60201-1-PBS)

    IHC staining of human lymphoma using 60201-1-Ig (same clone as 60201-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60201-1-Ig (UBC9 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60201-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBE2I/UBC9 antibody included
Planning an IHC experiment? We recommend our IHCeasy UBE2I/UBC9 Ready-To-Use IHC Kit. UBE2I/UBC9 primary antibody included.

产品信息

60201-1-PBS targets UBC9 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen UBC9 fusion protein Ag7885 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
Synonyms C358B7.1, E2,UBE2I, P18, SUMO conjugating enzyme UBC9, SUMO protein ligase, UBC9, UBCE9, UBE2I, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin protein ligase I
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC004437
Gene Symbol UBC9
Gene ID (NCBI) 7329
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP63279
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UBC9 is also named as UBE2I, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBC9 is detected in kidney and liver. Low amount of UBC9 is observed in brain, whereas the 18 kDa band of UBC9 is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBC9 antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).