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  • Featured Product
  • KD/KO Validated

UBE2C Monoclonal antibody, PBS Only

UBE2C Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C12H8

Cat no : 66087-1-PBS

Print datasheet

Synonyms

UBCH10, UBE2C, Ubiquitin carrier protein C, Ubiquitin protein ligase C

验证数据展示

  • IHC staining of human lung cancer using 66087-1-Ig (same clone as 66087-1-PBS)

    IHC staining of human lung cancer using 66087-1-Ig (same clone as 66087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66087-1-Ig (UBE2C antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66087-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBE2C antibody included
  • IHC staining of human lung cancer using 66087-1-Ig (same clone as 66087-1-PBS)

    IHC staining of human lung cancer using 66087-1-Ig (same clone as 66087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66087-1-Ig (UBE2C antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66087-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBE2C antibody included
  • WB analysis using 66087-1-Ig (same clone as 66087-1-PBS)

    WB analysis using 66087-1-Ig (same clone as 66087-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66087-1-Ig (UBE2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66087-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66087-1-Ig (same clone as 66087-1-PBS)

    IF Staining of HeLa using 66087-1-Ig (same clone as 66087-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBE2C antibody (66087-1-Ig, Clone: 1C12H8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66087-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy UBE2C Ready-To-Use IHC Kit. UBE2C primary antibody included.

产品信息

66087-1-PBS targets UBE2C in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen UBE2C fusion protein Ag19235 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ubiquitin-conjugating enzyme E2C
Synonyms UBCH10, UBE2C, Ubiquitin carrier protein C, Ubiquitin protein ligase C
Calculated Molecular Weight 179 aa, 20 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC016292
Gene Symbol UBE2C
Gene ID (NCBI) 11065
RRIDAB_11232220
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00762
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UBE2C(Ubiquitin-conjugating enzyme E2 C) is also known as UBCH10 and belongs to the ubiquitin-conjugating enzyme family. It is highly expressed in a variety of human primary tumors, and it is able to promote cell proliferation and malignant transformation(PMID:18703417). UBE2C, along with the E3 ligase of the anaphasepromoting complex (APC), catalyzes the ubiquitination of mitotic cyclins A and B, as well as securin. UBE2C is essential for cell cycle progression, and mutation of its active site cysteine confers a dominant-negative phenotype(PMID:17217624).