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  • Featured Product
  • KD/KO Validated

UBE2C Monoclonal antibody, PBS Only

UBE2C Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1C12H8

Cat No : 66087-1-PBS

Print datasheet

Synonyms

UBCH10, UBE2C, Ubiquitin carrier protein C, Ubiquitin protein ligase C

验证数据展示

  • IHC staining of human lung cancer using 66087-1-Ig (same clone as 66087-1-PBS)

    IHC staining of human lung cancer using 66087-1-Ig (same clone as 66087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66087-1-Ig (UBE2C antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66087-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBE2C antibody included
  • IHC staining of human lung cancer using 66087-1-Ig (same clone as 66087-1-PBS)

    IHC staining of human lung cancer using 66087-1-Ig (same clone as 66087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66087-1-Ig (UBE2C antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66087-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBE2C antibody included
  • WB analysis using 66087-1-Ig (same clone as 66087-1-PBS)

    WB analysis using 66087-1-Ig (same clone as 66087-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66087-1-Ig (UBE2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66087-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66087-1-Ig (same clone as 66087-1-PBS)

    IF Staining of HeLa using 66087-1-Ig (same clone as 66087-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBE2C antibody (66087-1-Ig, Clone: 1C12H8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66087-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy UBE2C Ready-To-Use IHC Kit. UBE2C primary antibody included.

产品信息

66087-1-PBS targets UBE2C in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity human, mouse, rat
Immunogen UBE2C fusion protein Ag19235 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ubiquitin-conjugating enzyme E2C
Synonyms UBCH10, UBE2C, Ubiquitin carrier protein C, Ubiquitin protein ligase C
Calculated Molecular Weight 179 aa, 20 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC016292
Gene Symbol UBE2C
Gene ID (NCBI) 11065
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00762
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

UBE2C(Ubiquitin-conjugating enzyme E2 C) is also known as UBCH10 and belongs to the ubiquitin-conjugating enzyme family. It is highly expressed in a variety of human primary tumors, and it is able to promote cell proliferation and malignant transformation(PMID:18703417). UBE2C, along with the E3 ligase of the anaphasepromoting complex (APC), catalyzes the ubiquitination of mitotic cyclins A and B, as well as securin. UBE2C is essential for cell cycle progression, and mutation of its active site cysteine confers a dominant-negative phenotype(PMID:17217624).