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UBE2C Monoclonal antibody

UBE2C Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

CloneNo.

1C12H8

Cat no : 66087-1-Ig

Print datasheet

Synonyms

UBCH10, UBE2C, Ubiquitin carrier protein C, Ubiquitin protein ligase C



经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, U2OS cells, A549 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy UBE2C Ready-To-Use IHC Kit. UBE2C primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 66087-1-Ig is UBE2C Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, CoIP, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen UBE2C fusion protein Ag19235 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ubiquitin-conjugating enzyme E2C
Synonyms UBCH10, UBE2C, Ubiquitin carrier protein C, Ubiquitin protein ligase C
Calculated Molecular Weight 179 aa, 20 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC016292
Gene Symbol UBE2C
Gene ID (NCBI) 11065
RRIDAB_11232220
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00762
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBE2C(Ubiquitin-conjugating enzyme E2 C) is also known as UBCH10 and belongs to the ubiquitin-conjugating enzyme family. It is highly expressed in a variety of human primary tumors, and it is able to promote cell proliferation and malignant transformation(PMID:18703417). UBE2C, along with the E3 ligase of the anaphasepromoting complex (APC), catalyzes the ubiquitination of mitotic cyclins A and B, as well as securin. UBE2C is essential for cell cycle progression, and mutation of its active site cysteine confers a dominant-negative phenotype(PMID:17217624).

实验方案

Product Specific Protocols
WB protocol for UBE2C antibody 66087-1-IgDownload protocol
IHC protocol for UBE2C antibody 66087-1-IgDownload protocol
IF protocol for UBE2C antibody 66087-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cell Rep Med

Distinctive multicellular immunosuppressive hubs confer different intervention strategies for left- and right-sided colon cancers

Authors - Bingxin Liu
humanWB

EMBO J

Shared and distinct mechanisms of UBA1 inactivation across different diseases

Authors - Jason C Collins
WB

Elife

Renal medullary carcinomas depend upon SMARCB1 loss and are sensitive to proteasome inhibition.

Authors - Andrew L Hong
humanIHC,IF

Cancer Lett

LINC01194 recruits NUMA1 to promote ubiquitination of RYR2 to enhance malignant progression in triple-negative breast cancer.

Authors - Duanyang Zhai
humanWB

Int J Biol Sci

PRPF6 promotes androgen receptor/androgen receptor-variant 7 actions in castration-resistant prostate cancer cells.

Authors - Wei Liu
humanWB,IHC

FEBS J

Silencing ubiquitin-conjugating enzyme 2C inhibits proliferation and epithelial-mesenchymal transition in pancreatic ductal adenocarcinoma.

Authors - Xianxing Wang
  • KD Validated