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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated UBE2C Monoclonal antibody

UBE2C Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C12H8

Cat no : CL488-66087

Print datasheet

Synonyms

UB E2C, EC:2.3.2.24, EC:2.3.2.23, E2 ubiquitin-conjugating enzyme C, 1C12H8

验证数据展示

  • IF Staining of HeLa using CL488-66087

    IF Staining of HeLa using CL488-66087

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 UBE2C antibody (CL488-66087, Clone: 1C12H8 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-66087

    FC experiment of HeLa using CL488-66087

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human UBE2C (CL488-66087, Clone:1C12H8) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy UBE2C Ready-To-Use IHC Kit. UBE2C primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66087-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66087 targets UBE2C in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen UBE2C fusion protein Ag19235 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ubiquitin-conjugating enzyme E2C
Synonyms UB E2C, EC:2.3.2.24, EC:2.3.2.23, E2 ubiquitin-conjugating enzyme C, 1C12H8
Calculated Molecular Weight 179 aa, 20 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC016292
Gene Symbol UBE2C
Gene ID (NCBI) 11065
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO00762
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBE2C(Ubiquitin-conjugating enzyme E2 C) is also known as UBCH10 and belongs to the ubiquitin-conjugating enzyme family. It is highly expressed in a variety of human primary tumors, and it is able to promote cell proliferation and malignant transformation(PMID:18703417). UBE2C, along with the E3 ligase of the anaphasepromoting complex (APC), catalyzes the ubiquitination of mitotic cyclins A and B, as well as securin. UBE2C is essential for cell cycle progression, and mutation of its active site cysteine confers a dominant-negative phenotype(PMID:17217624).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 UBE2C antibody CL488-66087Download protocol
FC protocol for CL Plus 488 UBE2C antibody CL488-66087Download protocol
Standard Protocols
Click here to view our Standard Protocols