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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68138-1-Ig (same clone as 68138-1-PBS)

    WB analysis using 68138-1-Ig (same clone as 68138-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68138-1-Ig (UBE2D3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68138-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 68138-1-Ig (same clone as 68138-1-PBS)

    IF Staining of A431 using 68138-1-Ig (same clone as 68138-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using UBE2D1/2/3/4 antibody (68138-1-Ig, Clone: 1C7D12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68138-1-PBS in a different storage buffer formulation.

UBE2D1/2/3/4 Monoclonal antibody, PBS Only

UBE2D1/2/3/4 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68138-1-PBS

产品说明书

CloneNo. 1C7D12

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

WB, IF/ICC, Indirect ELISA

别名

E2(17)KB3, UBC4/5, UBC5C, UBCH5C, UBE2D3, Ubiquitin carrier protein D3, Ubiquitin protein ligase D3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68138-1-PBS targets UBE2D1/2/3/4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 UBE2D1/2/3/4 fusion protein Ag2278 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast)
别名 E2(17)KB3, UBC4/5, UBC5C, UBCH5C, UBE2D3, Ubiquitin carrier protein D3, Ubiquitin protein ligase D3
计算分子量 17 kDa
观测分子量17 kDa
GenBank蛋白编号BC003395
基因名称 UBE2D3
Gene ID (NCBI) 7323
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP61077
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ubiquitination is a post-translational modification pathway or is part of the specific protein-degradation pathway by the 26S proteasome. Ubiquitination of a target protein involves multistep enzymatic reaction catalyzed by a cascade of enzymes including Ub-activating enzymes (E1s), Ub-conjugating enzymes (E2s) and Ub ligases (E3s) (PMID: 23542885). UBE2D family, which has 4 members named as UBE2D1/2/3/4, is an E2 ubiquitin-conjugating enzyme family in the ubiquitin-proteasome system. This antibody can recognize all the four members of UBE2D due to the high homology.