CoraLite® Plus 488-conjugated UBE2D1/2/3/4 Monoclonal antibody
UBE2D1/2/3/4 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1C7D12
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | A431 cells |
For other applications, we recommend the unconjugated version of this antibody, 68138-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68138 targets UBE2D1/2/3/4 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | Human, Mouse, Rat |
| Immunogen | UBE2D1/2/3/4 fusion protein Ag2278 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast) |
| Synonyms | E2(17)KB3, UBC4/5, UBC5C, UBCH5C, UBE2D3, Ubiquitin carrier protein D3, Ubiquitin protein ligase D3 |
| Calculated Molecular Weight | 17 kDa |
| GenBank Accession Number | BC003395 |
| Gene Symbol | UBE2D3 |
| Gene ID (NCBI) | 7323 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P61077 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ubiquitination is a post-translational modification pathway or is part of the specific protein-degradation pathway by the 26S proteasome. Ubiquitination of a target protein involves multistep enzymatic reaction catalyzed by a cascade of enzymes including Ub-activating enzymes (E1s), Ub-conjugating enzymes (E2s) and Ub ligases (E3s) (PMID: 23542885). UBE2D family, which has 4 members named as UBE2D1/2/3/4, is an E2 ubiquitin-conjugating enzyme family in the ubiquitin-proteasome system. This antibody can recognize all the four members of UBE2D due to the high homology.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 UBE2D1/2/3/4 antibody CL488-68138 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |