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UBE2S Recombinant antibody, PBS Only

UBE2S Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6D18

Cat no : 81854-1-PBS

Print datasheet

Synonyms

EPF5, EC:2.3.2.23, E2-EPF, E2EPF, E2 EPF

验证数据展示

  • WB analysis using 81854-1-RR (same clone as 81854-1-PBS)

    WB analysis using 81854-1-RR (same clone as 81854-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81854-1-RR (UBE2S antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81854-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 81854-1-RR (same clone as 81854-1-PBS)

    IHC staining of mouse testis using 81854-1-RR (same clone as 81854-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 81854-1-RR (UBE2S antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 81854-1-PBS in a different storage buffer formulation.

  • IF Staining of NCCIT using 81854-1-RR (same clone as 81854-1-PBS)

    IF Staining of NCCIT using 81854-1-RR (same clone as 81854-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NCCIT cells using UBE2S antibody (81854-1-RR, Clone: 6D18 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81854-1-PBS in a different storage buffer formulation.

产品信息

81854-1-PBS targets UBE2S in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen UBE2S fusion protein Ag5264 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ubiquitin-conjugating enzyme E2S
Synonyms EPF5, EC:2.3.2.23, E2-EPF, E2EPF, E2 EPF
Calculated Molecular Weight 222 aa, 24 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC066948
Gene Symbol UBE2S
Gene ID (NCBI) 27338
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16763
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UBE2S(Ubiquitin-conjugating enzyme E2 S) is also named as E2EPF and belongs to the ubiquitin-conjugating enzyme family. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C) and a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Overexpression of UBE2S can increase tumor cell proliferation, invasion, and metastasis through the VHL-HIF pathway (PMID:16819549).