• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

UBE2S Recombinant antibody, PBS Only

UBE2S Recombinant Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

6D18

Cat No : 81854-1-PBS

Print datasheet

Synonyms

E2 EPF, E2EPF, EPF5, UBE2S, Ubiquitin carrier protein S, Ubiquitin protein ligase S

验证数据展示

  • WB analysis using 81854-1-RR (same clone as 81854-1-PBS)

    WB analysis using 81854-1-RR (same clone as 81854-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81854-1-RR (UBE2S antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81854-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 81854-1-RR (same clone as 81854-1-PBS)

    IHC staining of mouse testis using 81854-1-RR (same clone as 81854-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 81854-1-RR (UBE2S antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 81854-1-PBS in a different storage buffer formulation.

  • IF Staining of NCCIT using 81854-1-RR (same clone as 81854-1-PBS)

    IF Staining of NCCIT using 81854-1-RR (same clone as 81854-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NCCIT cells using UBE2S antibody (81854-1-RR, Clone: 6D18 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81854-1-PBS in a different storage buffer formulation.

产品信息

81854-1-PBS targets UBE2S in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, mouse
Immunogen UBE2S fusion protein Ag5264 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ubiquitin-conjugating enzyme E2S
Synonyms E2 EPF, E2EPF, EPF5, UBE2S, Ubiquitin carrier protein S, Ubiquitin protein ligase S
Calculated Molecular Weight 222 aa, 24 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC066948
Gene Symbol UBE2S
Gene ID (NCBI) 27338
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16763
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

UBE2S(Ubiquitin-conjugating enzyme E2 S) is also named as E2EPF and belongs to the ubiquitin-conjugating enzyme family. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C) and a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Overexpression of UBE2S can increase tumor cell proliferation, invasion, and metastasis through the VHL-HIF pathway (PMID:16819549).