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验证数据展示

  • WB analysis using 81854-1-RR

    WB analysis using 81854-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81854-1-RR (UBE2S antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 81854-1-RR

    IHC staining of mouse testis using 81854-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 81854-1-RR (UBE2S antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

  • IF Staining of NCCIT using 81854-1-RR

    IF Staining of NCCIT using 81854-1-RR

    Immunofluorescent analysis of (4% PFA) fixed NCCIT cells using UBE2S antibody (81854-1-RR, Clone: 6D18 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 81854-1-RR

    Affinity and Kinetic Characterization of 81854-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81854-1-RR against Human UBE2S were performed. The affinity constant is below 1 pM.

UBE2S Recombinant antibody

UBE2S Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 81854-1-RR

Print datasheet

CloneNo. 6D18

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

6D18, E2 EPF, E2EPF, E2-EPF, EC:2.3.2.23

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, HepG2 cells, K-562 cells, NIH/3T3 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNCCIT cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81854-1-RR targets UBE2S in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen UBE2S fusion protein Ag5264 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ubiquitin-conjugating enzyme E2S
Synonyms 6D18, E2 EPF, E2EPF, E2-EPF, EC:2.3.2.23
Calculated Molecular Weight 222 aa, 24 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC066948
Gene Symbol UBE2S
Gene ID (NCBI) 27338
RRIDAB_2935586
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16763
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBE2S(Ubiquitin-conjugating enzyme E2 S) is also named as E2EPF and belongs to the ubiquitin-conjugating enzyme family. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C) and a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Overexpression of UBE2S can increase tumor cell proliferation, invasion, and metastasis through the VHL-HIF pathway (PMID:16819549).

实验方案

Product Specific Protocols
WB protocol for UBE2S antibody 81854-1-RRDownload protocol
IHC protocol for UBE2S antibody 81854-1-RRDownload protocol
IF protocol for UBE2S antibody 81854-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols