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  • KD/KO Validated

UBR5 Monoclonal antibody

UBR5 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (2)

Applications

IHC, WB, ELISA and More (2)

Conjugate

Unconjugated

CloneNo.

2E7A6

Cat No : 66937-1-Ig

Print datasheet

Synonyms

DD5, EDD, EDD1, FLJ11310, hHYD, HYD, KIAA0896, Progestin induced protein, UBR5



经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells, PC-12 cells, HEK-293 cells, Jurkat cells, Neuro-2a cells
Positive IHC detected inmouse testis tissue, mouse brain tissue, rat testis tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy UBR5 Ready-To-Use IHC Kit. UBR5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66937-1-Ig targets UBR5 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IHC, WB, ELISA Application Description
Cited ApplicationsWB, IF, IHC, CoIP
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, Ae. aegypti
Immunogen UBR5 fusion protein Ag18753 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ubiquitin protein ligase E3 component n-recognin 5
Synonyms DD5, EDD, EDD1, FLJ11310, hHYD, HYD, KIAA0896, Progestin induced protein, UBR5
Calculated Molecular Weight 2799 aa, 309 kDa
Observed Molecular Weight 309 kDa
GenBank Accession NumberBC137234
Gene Symbol UBR5
Gene ID (NCBI) 51366
RRIDAB_2882261
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO95071
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBR5, also named as ubiquitin protein ligase E3 component n-reconin5, is a HECT domain-containing protein as a myocardin-binding protein. UBR5 is highly conserved in metazoans, has unique structural features, and has been implicated in regulation of DNA damage response, metabolism, transcription, and apoptosis. Hence, UBR5 is a key regulator of cell signaling relevant to broad areas of cancer biology (PMID: 26464214). The human UBR5 gene has 59 exons, encoding an approximately 10kb mRNA and >300 kDa protein with widespread expression in various cell types.

实验方案

Product Specific Protocols
WB protocol for UBR5 antibody 66937-1-IgDownload protocol
IHC protocol for UBR5 antibody 66937-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
Ae. aegyptiWB

Nat Commun

Analysis of Zika virus capsid-Aedes aegypti mosquito interactome reveals pro-viral host factors critical for establishing infection.

Authors - Rommel J Gestuveo
  • KD Validated
humanWB,IF

Exp Eye Res

Retinitis pigmentosa 2 pathogenic mutants degrade through BAG6/HUWE1 complex.

Authors - Jing Zhang
  • KD Validated
humanWB,IHC

Cell Death Dis

CSN6 promotes melanoma proliferation and metastasis by controlling the UBR5-mediated ubiquitination and degradation of CDK9.

Authors - Yanli Zhang
  • KD Validated
humanWB

Adv Sci (Weinh)

Phosphorylated SHMT2 Regulates Oncogenesis Through m6 A Modification in Lung Adenocarcinoma

Authors - Tianyu Han
humanWB,CoIP

J Virol

Multiple E3 ligases act as antiviral factors against SARS-CoV-2 via inducing the ubiquitination and degradation of ORF9b

Authors - Miao Yu
  • KD Validated