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验证数据展示

  • WB analysis using 66937-1-Ig (same clone as 66937-1-PBS)

    WB analysis using 66937-1-Ig (same clone as 66937-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66937-1-Ig (same clone as 66937-1-PBS)

    WB analysis of HeLa using 66937-1-Ig (same clone as 66937-1-PBS)

    WB result of UBR5 antibody (66937-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBR5 transfected HeLa cells. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 66937-1-Ig (same clone as 66937-1-PBS)

    IHC staining of mouse testis using 66937-1-Ig (same clone as 66937-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBR5 antibody included
  • IHC staining of mouse testis using 66937-1-Ig (same clone as 66937-1-PBS)

    IHC staining of mouse testis using 66937-1-Ig (same clone as 66937-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBR5 antibody included
  • Featured Product
  • KD/KO验证

UBR5 Monoclonal antibody, PBS Only

UBR5 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66937-1-PBS

产品说明书

CloneNo. 2E7A6

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

DD5, EDD, EDD1, FLJ11310, hHYD, HYD, KIAA0896, Progestin induced protein, UBR5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy UBR5 Ready-To-Use IHC Kit. UBR5 primary antibody included.

产品信息

66937-1-PBS targets UBR5 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 UBR5 fusion protein Ag18753 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquitin protein ligase E3 component n-recognin 5
别名 DD5, EDD, EDD1, FLJ11310, hHYD, HYD, KIAA0896, Progestin induced protein, UBR5
计算分子量 2799 aa, 309 kDa
观测分子量 309 kDa
GenBank蛋白编号BC137234
基因名称 UBR5
Gene ID (NCBI) 51366
RRIDAB_2882261
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO95071
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UBR5, also named as ubiquitin protein ligase E3 component n-reconin5, is a HECT domain-containing protein as a myocardin-binding protein. UBR5 is highly conserved in metazoans, has unique structural features, and has been implicated in regulation of DNA damage response, metabolism, transcription, and apoptosis. Hence, UBR5 is a key regulator of cell signaling relevant to broad areas of cancer biology (PMID: 26464214). The human UBR5 gene has 59 exons, encoding an approximately 10kb mRNA and >300 kDa protein with widespread expression in various cell types.