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  • Featured Product
  • KD/KO Validated

UBR5 Monoclonal antibody, PBS Only

UBR5 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E7A6

Cat no : 66937-1-PBS

Print datasheet

Synonyms

DD5, EDD, EDD1, FLJ11310, hHYD, HYD, KIAA0896, Progestin induced protein, UBR5

验证数据展示

  • WB analysis using 66937-1-Ig (same clone as 66937-1-PBS)

    WB analysis using 66937-1-Ig (same clone as 66937-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66937-1-Ig (same clone as 66937-1-PBS)

    WB analysis of HeLa using 66937-1-Ig (same clone as 66937-1-PBS)

    WB result of UBR5 antibody (66937-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBR5 transfected HeLa cells. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 66937-1-Ig (same clone as 66937-1-PBS)

    IHC staining of mouse testis using 66937-1-Ig (same clone as 66937-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBR5 antibody included
  • IHC staining of mouse testis using 66937-1-Ig (same clone as 66937-1-PBS)

    IHC staining of mouse testis using 66937-1-Ig (same clone as 66937-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBR5 antibody included
Planning an IHC experiment? We recommend our IHCeasy UBR5 Ready-To-Use IHC Kit. UBR5 primary antibody included.

产品信息

66937-1-PBS targets UBR5 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen UBR5 fusion protein Ag18753 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ubiquitin protein ligase E3 component n-recognin 5
Synonyms DD5, EDD, EDD1, FLJ11310, hHYD, HYD, KIAA0896, Progestin induced protein, UBR5
Calculated Molecular Weight 2799 aa, 309 kDa
Observed Molecular Weight 309 kDa
GenBank Accession NumberBC137234
Gene Symbol UBR5
Gene ID (NCBI) 51366
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO95071
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UBR5, also named as ubiquitin protein ligase E3 component n-reconin5, is a HECT domain-containing protein as a myocardin-binding protein. UBR5 is highly conserved in metazoans, has unique structural features, and has been implicated in regulation of DNA damage response, metabolism, transcription, and apoptosis. Hence, UBR5 is a key regulator of cell signaling relevant to broad areas of cancer biology (PMID: 26464214). The human UBR5 gene has 59 exons, encoding an approximately 10kb mRNA and >300 kDa protein with widespread expression in various cell types.