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UCHL3 Recombinant antibody, PBS Only

UCHL3 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242015A10

Cat no : 84542-6-PBS

Print datasheet

Synonyms

UCH-L3, UCH L3, Ubiquitin thioesterase L3, Ubiquitin carboxyl terminal hydrolase isozyme L3, EC:3.4.19.12

验证数据展示

  • WB analysis using 84542-6-RR (same clone as 84542-6-PBS)

    WB analysis using 84542-6-RR (same clone as 84542-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84542-6-RR (UCHL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84542-6-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 84542-6-RR (same clone as 84542-6-PBS)

    IHC staining of mouse testis using 84542-6-RR (same clone as 84542-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84542-6-RR (UCHL3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84542-6-PBS in a different storage buffer formulation.

产品信息

The immunogen of 84542-6-PBS is UCHL3 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, rat
Immunogen UCHL3 fusion protein Ag3053 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
Synonyms UCH-L3, UCH L3, Ubiquitin thioesterase L3, Ubiquitin carboxyl terminal hydrolase isozyme L3, EC:3.4.19.12
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC018125
Gene Symbol UCHL3
Gene ID (NCBI) 7347
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP15374
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UCH-L3 belongs to the ubiquitin C-terminal hydrolase family that deubiquitinates ubiquitin-protein conjugates in the ubiquitin-proteasome system. This enzyme indirectly increases the phosphorylation of IGFIR, AKT, FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. It is required for stress-response retinal, skeletal muscle and germ cell maintenance and may be involved in working memory.