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UCHL3 Recombinant antibody, PBS Only

UCHL3 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242015A10

Cat no : 84542-6-PBS

Print datasheet

Synonyms

UCH-L3, UCH L3, Ubiquitin thioesterase L3, Ubiquitin carboxyl terminal hydrolase isozyme L3, EC:3.4.19.12

验证数据展示

  • WB analysis using 84542-6-RR (same clone as 84542-6-PBS)

    WB analysis using 84542-6-RR (same clone as 84542-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84542-6-RR (UCHL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84542-6-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 84542-6-RR (same clone as 84542-6-PBS)

    IHC staining of mouse testis using 84542-6-RR (same clone as 84542-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84542-6-RR (UCHL3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84542-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84542-6-RR

    Affinity and Kinetic Characterization of 84542-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84542-6-RR against Human UCHL3 were performed. The affinity constant is 2.45 nM.

产品信息

84542-6-PBS targets UCHL3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, rat
Immunogen UCHL3 fusion protein Ag3053 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
Synonyms UCH-L3, UCH L3, Ubiquitin thioesterase L3, Ubiquitin carboxyl terminal hydrolase isozyme L3, EC:3.4.19.12
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC018125
Gene Symbol UCHL3
Gene ID (NCBI) 7347
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP15374
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UCH-L3 belongs to the ubiquitin C-terminal hydrolase family that deubiquitinates ubiquitin-protein conjugates in the ubiquitin-proteasome system. This enzyme indirectly increases the phosphorylation of IGFIR, AKT, FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. It is required for stress-response retinal, skeletal muscle and germ cell maintenance and may be involved in working memory.