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×
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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84542-6-RR (same clone as 84542-6-PBS)

    WB analysis using 84542-6-RR (same clone as 84542-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84542-6-RR (UCHL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84542-6-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 84542-6-RR (same clone as 84542-6-PBS)

    IHC staining of mouse testis using 84542-6-RR (same clone as 84542-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84542-6-RR (UCHL3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84542-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84542-6-RR

    Affinity and Kinetic Characterization of 84542-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84542-6-RR against Human UCHL3 were performed. The affinity constant is 2.45 nM.

UCHL3 Recombinant antibody, PBS Only

UCHL3 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84542-6-PBS

产品说明书

CloneNo. 242015A10

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, Indirect ELISA

别名

UCH-L3, UCH L3, Ubiquitin thioesterase L3, Ubiquitin carboxyl terminal hydrolase isozyme L3, EC:3.4.19.12

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84542-6-PBS targets UCHL3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 UCHL3 fusion protein Ag3053 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
别名 UCH-L3, UCH L3, Ubiquitin thioesterase L3, Ubiquitin carboxyl terminal hydrolase isozyme L3, EC:3.4.19.12
计算分子量 26 kDa
观测分子量 26 kDa
GenBank蛋白编号BC018125
基因名称 UCHL3
Gene ID (NCBI) 7347
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP15374
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UCH-L3 belongs to the ubiquitin C-terminal hydrolase family that deubiquitinates ubiquitin-protein conjugates in the ubiquitin-proteasome system. This enzyme indirectly increases the phosphorylation of IGFIR, AKT, FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. It is required for stress-response retinal, skeletal muscle and germ cell maintenance and may be involved in working memory.