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验证数据展示

  • WB analysis using 84542-6-RR

    WB analysis using 84542-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84542-6-RR (UCHL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 84542-6-RR

    IHC staining of mouse testis using 84542-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84542-6-RR (UCHL3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84542-6-RR

    Affinity and Kinetic Characterization of 84542-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84542-6-RR against Human UCHL3 were performed. The affinity constant is 2.45 nM.

UCHL3 Recombinant antibody

UCHL3 Recombinant Antibody for WB, IHC, ELISA
Cat No. 84542-6-RR

产品说明书

CloneNo. 242015A10

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, ELISA

别名

UCH-L3, UCH L3, Ubiquitin thioesterase L3, Ubiquitin carboxyl terminal hydrolase isozyme L3, EC:3.4.19.12

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, mouse spleen tissue, MCF-7 cells, Jurkat cells, SH-SY5Y cells, BxPC-3 cells, rat spleen tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84542-6-RR targets UCHL3 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, rat
免疫原 UCHL3 fusion protein Ag3053 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
别名 UCH-L3, UCH L3, Ubiquitin thioesterase L3, Ubiquitin carboxyl terminal hydrolase isozyme L3, EC:3.4.19.12
计算分子量 26 kDa
观测分子量 26 kDa
GenBank蛋白编号BC018125
基因名称 UCHL3
Gene ID (NCBI) 7347
RRIDAB_3672047
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP15374
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UCH-L3 belongs to the ubiquitin C-terminal hydrolase family that deubiquitinates ubiquitin-protein conjugates in the ubiquitin-proteasome system. This enzyme indirectly increases the phosphorylation of IGFIR, AKT, FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. It is required for stress-response retinal, skeletal muscle and germ cell maintenance and may be involved in working memory.

实验方案

Product Specific Protocols
WB protocol for UCHL3 antibody 84542-6-RRDownload protocol
IHC protocol for UCHL3 antibody 84542-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols