验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, human adipose tissue, human skeletal muscle tissue |
It is strongly recommended that fresh-made lysate be used for western blot assays.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
66700-1-Ig targets UCP2 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Immunogen | UCP2 fusion protein Ag19361 种属同源性预测 |
| Host / Isotype | Mouse / IgG3 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | uncoupling protein 2 (mitochondrial, proton carrier) |
| Synonyms | BMIQ4, SLC25A8, UCP 2, UCP2, UCPH |
| Calculated Molecular Weight | 33 kDa |
| Observed Molecular Weight | 28 kDa |
| GenBank Accession Number | BC011737 |
| Gene Symbol | UCP2 |
| Gene ID (NCBI) | 7351 |
| RRID | AB_2882053 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P55851 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UCP2 (uncoupling protein 2) is a member of the mitochondrial carrier protein super family located in the mitochondrial inner membrane. The UCPs differ greatly in tissue distribution: UCP1 is expressed only in brown adipose; UCP3 preferentially in skeletal muscle, brown adipose and heart; UCP4 and UCP5 mainly in the nervous system (18167556). In contract, UCP2 has a broad distribution and is implicated in a wide range of pathophysiological processes, including diabetes and cancer. This antibody detect a band around 28-33 kDa corresponding to UCP2 protein in mouse liver, skeletal muscle and pancreas. Some upper and lower bands also were observed due to the dimer formation and degradation of the UCP2 protein (20103532). This antibody was raised against the full-length UCP2 protein and may cross-react with UCP3. It is notable that UCP2 is known to have a very short half-life less than 1 hour (17240372). It is strongly recommended that fresh-made lysate be used for western blot assays.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for UCP2 antibody 66700-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |