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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of Jurkat using 66700-1-Ig (same clone as 66700-1-PBS)

    WB analysis of Jurkat using 66700-1-Ig (same clone as 66700-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66700-1-Ig (UCP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66700-1-PBS in a different storage buffer formulation.

  • WB analysis of human adipose using 66700-1-Ig (same clone as 66700-1-PBS)

    WB analysis of human adipose using 66700-1-Ig (same clone as 66700-1-PBS)

    human adipose tissue were subjected to SDS PAGE followed by western blot with 66700-1-Ig (UCP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66700-1-PBS in a different storage buffer formulation.

  • WB analysis of human skeletal muscle using 66700-1-Ig (same clone as 66700-1-PBS)

    WB analysis of human skeletal muscle using 66700-1-Ig (same clone as 66700-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66700-1-Ig (UCP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66700-1-PBS in a different storage buffer formulation.

UCP2 Monoclonal antibody, PBS Only

UCP2 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66700-1-PBS

产品说明书

CloneNo. 2B4B1

宿主/亚型

Mouse / IgG3

种属反应性

Human

应用

WB, Indirect ELISA

别名

BMIQ4, SLC25A8, UCP 2, UCP2, UCPH

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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It is strongly recommended that fresh-made lysate be used for western blot assays.

Planning an IHC experiment? We recommend our IHCeasy UCP2 Ready-To-Use IHC Kit. UCP2 primary antibody included.

产品信息

66700-1-PBS targets UCP2 in WB, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 Human
免疫原 UCP2 fusion protein Ag19361 种属同源性预测
宿主/亚型 Mouse / IgG3
抗体类别 Monoclonal
产品类型 Antibody
全称 uncoupling protein 2 (mitochondrial, proton carrier)
别名 BMIQ4, SLC25A8, UCP 2, UCP2, UCPH
计算分子量 33 kDa
观测分子量 28 kDa
GenBank蛋白编号BC011737
基因名称 UCP2
Gene ID (NCBI) 7351
RRIDAB_2882053
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP55851
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UCP2 (uncoupling protein 2) is a member of the mitochondrial carrier protein super family located in the mitochondrial inner membrane. The UCPs differ greatly in tissue distribution: UCP1 is expressed only in brown adipose; UCP3 preferentially in skeletal muscle, brown adipose and heart; UCP4 and UCP5 mainly in the nervous system (18167556). In contract, UCP2 has a broad distribution and is implicated in a wide range of pathophysiological processes, including diabetes and cancer. This antibody detect a band around 28-33 kDa corresponding to UCP2 protein in mouse liver, skeletal muscle and pancreas. Some upper and lower bands also were observed due to the dimer formation and degradation of the UCP2 protein (20103532). This antibody was raised against the full-length UCP2 protein and may cross-react with UCP3. It is notable that UCP2 is known to have a very short half-life less than 1 hour (17240372). It is strongly recommended that fresh-made lysate be used for western blot assays.