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  • Featured Product
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CoraLite® Plus 488-conjugated UGDH Polyclonal antibody

UGDH Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-13151

Print datasheet

Synonyms

GDH, UDP Glc dehydrogenase, UDP GlcDH, UDP glucose 6 dehydrogenase, UDP glucose dehydrogenase, UDPGDH, UGD, UGDH

验证数据展示

  • IF Staining of HepG2 using CL488-13151

    IF Staining of HepG2 using CL488-13151

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 UGDH antibody (CL488-13151) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 13151-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13151 targets UGDH in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen UGDH fusion protein Ag3767 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name UDP-glucose dehydrogenase
Synonyms GDH, UDP Glc dehydrogenase, UDP GlcDH, UDP glucose 6 dehydrogenase, UDP glucose dehydrogenase, UDPGDH, UGD, UGDH
Calculated Molecular Weight 494 aa, 55 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC022781
Gene Symbol UGDH
Gene ID (NCBI) 7358
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60701
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UDP-glucose 6-dehydrogenase (UGDH) is a key enzyme in the uronic acid pathway, and converts UDP-glucose (UDP-Glc) to UDP-glucuronic acid (UDP-GlcA). UGDH is critical to the production of extracellular matrix components which are essential to the migration and connectivity of neurons early in human brain development. UDP-GlcA is an essential sugar nucleotide precursor and a key component in the synthesis and stepwise degradation of glycosaminoglycans (GAGs). The protein subunit migrates on SDS-PAGE at ~55 kDa. (PMID: 32175296, PMID: 21576248, PMID: 31243371).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 UGDH antibody CL488-13151Download protocol
Standard Protocols
Click here to view our Standard Protocols