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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84489-4-RR (same clone as 84489-4-PBS)

    WB analysis using 84489-4-RR (same clone as 84489-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84489-4-RR (UHRF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84489-4-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293T using 84489-4-RR (same clone as 84489-4-PBS)

    WB analysis of HEK-293T using 84489-4-RR (same clone as 84489-4-PBS)

    WB result of UHRF1 antibody (84489-4-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UHRF1 transfected HEK-293T cells. This data was developed using the same antibody clone with 84489-4-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84489-4-RR (same clone as 84489-4-PBS)

    IF Staining of HepG2 using 84489-4-RR (same clone as 84489-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UHRF1 antibody (84489-4-RR, Clone: 241872B12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84489-4-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 84489-4-RR (same clone as 84489-4-PBS)

    FC experiment of U2OS using 84489-4-RR (same clone as 84489-4-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug UHRF1 Recombinant antibody (84489-4-RR, Clone:241872B12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84489-4-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

UHRF1 Recombinant antibody, PBS Only

UHRF1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84489-4-PBS

产品说明书

CloneNo. 241872B12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

241872B12, E3 ubiquitin-protein ligase UHRF1, EC:2.3.2.27, hNp95, hUHRF1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy UHRF1 Ready-To-Use IHC Kit. UHRF1 primary antibody included.

产品信息

84489-4-PBS targets UHRF1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 UHRF1 fusion protein Ag17498 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ubiquitin-like with PHD and ring finger domains 1
别名 241872B12, E3 ubiquitin-protein ligase UHRF1, EC:2.3.2.27, hNp95, hUHRF1
计算分子量 806 aa, 91 kDa
观测分子量100 kDa
GenBank蛋白编号BC113875
基因名称 UHRF1
Gene ID (NCBI) 29128
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ96T88
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UHRF1, also named as ICBP90, NP95 and RNF106, is a putative E3 ubiquitin-protein ligase. It may participate in methylation-dependent transcriptional regulation. UHRF1 binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. It is important for G1/S transition and may be involved in DNA repair and chromosomal stability. UHRF1's ability to repress its direct target gene expression is dependent on PHD(UHRF1) binding to unmodified H3R2. In addition, UHRF1 is a novel metabolic guardian restricting AMPK activity(PMID: 34907338).