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UHRF1 Recombinant antibody

UHRF1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241872B12

Cat no : 84489-4-RR

Print datasheet

Synonyms

HuNp95, hUHRF1, hNp95, EC:2.3.2.27, E3 ubiquitin-protein ligase UHRF1

验证数据展示

  • WB analysis using 84489-4-RR

    WB analysis using 84489-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84489-4-RR (UHRF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 84489-4-RR

    IF Staining of HepG2 using 84489-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UHRF1 antibody (84489-4-RR, Clone: 241872B12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of U2OS using 84489-4-RR

    FC experiment of U2OS using 84489-4-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug UHRF1 Recombinant antibody (84489-4-RR, Clone:241872B12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

经过测试的应用

Positive WB detected inMCF-7 cells, HuH-7 cells, HeLa cells, Jurkat cells, HepG2 cells, HCT-116 cells, U2OS cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy UHRF1 Ready-To-Use IHC Kit. UHRF1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84489-4-RR is UHRF1 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen UHRF1 fusion protein Ag17498 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ubiquitin-like with PHD and ring finger domains 1
Synonyms HuNp95, hUHRF1, hNp95, EC:2.3.2.27, E3 ubiquitin-protein ligase UHRF1
Calculated Molecular Weight 806 aa, 91 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC113875
Gene Symbol UHRF1
Gene ID (NCBI) 29128
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96T88
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UHRF1, also named as ICBP90, NP95 and RNF106, is a putative E3 ubiquitin-protein ligase. It may participate in methylation-dependent transcriptional regulation. UHRF1 binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. It is important for G1/S transition and may be involved in DNA repair and chromosomal stability. UHRF1's ability to repress its direct target gene expression is dependent on PHD(UHRF1) binding to unmodified H3R2. In addition, UHRF1 is a novel metabolic guardian restricting AMPK activity(PMID: 34907338).

实验方案

Product Specific Protocols
WB protocol for UHRF1 antibody 84489-4-RRDownload protocol
IF protocol for UHRF1 antibody 84489-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols