验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy UHRF1 Ready-To-Use IHC Kit. UHRF1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 84489-4-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-84489-4 targets UHRF1 in IF/ICC applications and shows reactivity with human samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human |
| Immunogen | UHRF1 fusion protein Ag17498 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | ubiquitin-like with PHD and ring finger domains 1 |
| Synonyms | |
| Calculated Molecular Weight | 806 aa, 91 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC113875 |
| Gene Symbol | UHRF1 |
| Gene ID (NCBI) | 29128 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96T88 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UHRF1, also named as ICBP90, NP95 and RNF106, is a putative E3 ubiquitin-protein ligase. It may participate in methylation-dependent transcriptional regulation. UHRF1 binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. It is important for G1/S transition and may be involved in DNA repair and chromosomal stability. UHRF1's ability to repress its direct target gene expression is dependent on PHD(UHRF1) binding to unmodified H3R2. In addition, UHRF1 is a novel metabolic guardian restricting AMPK activity(PMID: 34907338).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 UHRF1 antibody CL488-84489-4 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |