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ULK1 Monoclonal antibody

ULK1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D7G1

Cat no : 68445-1-Ig

Print datasheet

Synonyms

ATG1, FLJ38455, FLJ46475, KIAA0722, ULK1, Unc 51 like kinase 1, UNC51, Unc51.1

验证数据展示

  • WB analysis using 68445-1-Ig

    WB analysis using 68445-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 68445-1-Ig

    IHC staining of human liver using 68445-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ULK1 antibody included
  • IHC staining of human liver using 68445-1-Ig

    IHC staining of human liver using 68445-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ULK1 antibody included
  • IHC staining of mouse skeletal muscle using 68445-1-Ig

    IHC staining of mouse skeletal muscle using 68445-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ULK1 antibody included
  • IHC staining of mouse skeletal muscle using 68445-1-Ig

    IHC staining of mouse skeletal muscle using 68445-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ULK1 antibody included

经过测试的应用

Positive WB detected inA549 cells, PC-3 cells, HeLa cells, Jurkat cells, HT-1376 cells, LnCaP cells, T-47D cells, NIH/3T3 cells, PC-12 cells
Positive IHC detected inhuman liver tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ULK1 Ready-To-Use IHC Kit. ULK1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

68445-1-Ig targets ULK1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, Mouse, Rat
Cited Reactivitymouse
Immunogen ULK1 fusion protein Ag22397 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name unc-51-like kinase 1 (C. elegans)
Synonyms ATG1, FLJ38455, FLJ46475, KIAA0722, ULK1, Unc 51 like kinase 1, UNC51, Unc51.1
Observed Molecular Weight 130-150 kDa
GenBank Accession NumberNM_003565
Gene Symbol ULK1
Gene ID (NCBI) 8408
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75385
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ULK1, also named as KIAA0722, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and APG1/unc-51/ULK1 subfamily. It is involved in autophagy. ULK1 is required for autophagosome formation. It forms a stable complex with Atg13 and focal adhesion kinase (FAK) family interacting protein of 200 kDa (FIP 200)(PMID:21460634). ULK1 phosphorylates ATG13/KIAA0652. It is involved in axon growth. ULK1 plays an essential role in neurite extension of cerebellar granule cells. ULK1 represents a potential novel prognostic biomarker for HCC patients and may play an important role during the progression of HCC(PMID:23573318).

实验方案

Product Specific Protocols
WB protocol for ULK1 antibody 68445-1-IgDownload protocol
IHC protocol for ULK1 antibody 68445-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

ACS Infect Dis

Pec 1 of Pseudomonas aeruginosa Inhibits Bacterial Clearance of Host by Blocking Autophagy in Macrophages

Authors - Xiao Jin
View Article