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验证数据展示

  • IF Staining of HeLa using CL488-29005

    IF Staining of HeLa using CL488-29005

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 ULK1 antibody (CL488-29005) at dilution of 1:200.

CoraLite® Plus 488-conjugated ULK1 Polyclonal antibody

ULK1 Polyclonal Antibody for IF/ICC
Cat No. CL488-29005

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

Serine/threonine-protein kinase ULK1, KIAA0722, hATG1, EC:2.7.11.1, Autophagy-related protein 1 homolog

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ULK1 Ready-To-Use IHC Kit. ULK1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 29005-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-29005 targets ULK1 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 unc-51-like kinase 1 (C. elegans)
别名 Serine/threonine-protein kinase ULK1, KIAA0722, hATG1, EC:2.7.11.1, Autophagy-related protein 1 homolog
观测分子量130 kDa
GenBank蛋白编号NM_003565
基因名称 ULK1
Gene ID (NCBI) 8408
RRIDAB_3672835
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75385
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ULK1, also named as KIAA0722, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and APG1/unc-51/ULK1 subfamily. It is involved in autophagy. ULK1 is required for autophagosome formation. It forms a stable complex with Atg13 and focal adhesion kinase (FAK) family interacting protein of 200 kDa (FIP 200)(PMID:21460634). ULK1 phosphorylates ATG13/KIAA0652. It is involved in axon growth. ULK1 plays an essential role in neurite extension of cerebellar granule cells. ULK1 represents a potential novel prognostic biomarker for HCC patients and may play an important role during the progression of HCC(PMID:23573318).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ULK1 antibody CL488-29005Download protocol
Standard Protocols
Click here to view our Standard Protocols