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验证数据展示

  • WB analysis using 67193-1-Ig

    WB analysis using 67193-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67193-1-Ig (UNC13D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat spleen using 67193-1-Ig

    IHC staining of rat spleen using 67193-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 67193-1-Ig (UNC13D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UNC13D antibody included
  • IHC staining of rat spleen using 67193-1-Ig

    IHC staining of rat spleen using 67193-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 67193-1-Ig (UNC13D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UNC13D antibody included

UNC13D Monoclonal antibody

UNC13D Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67193-1-Ig

产品说明书

CloneNo. 1C5C1

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, pig

应用

WB, IHC, ELISA

别名

FHL3, HLH3, HPLH3, Munc13 4, Protein unc 13 homolog D, unc 13 homolog D (C. elegans), UNC13D

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inTHP-1 cells, RAW 264.7 cells, pig spleen tissue, rat spleen tissue, K-562 cells, Jurkat cells, HL-60 cells
Positive IHC detected inrat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy UNC13D Ready-To-Use IHC Kit. UNC13D primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67193-1-Ig targets UNC13D in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat, pig
免疫原 UNC13D fusion protein Ag10497 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 unc-13 homolog D (C. elegans)
别名 FHL3, HLH3, HPLH3, Munc13 4, Protein unc 13 homolog D, unc 13 homolog D (C. elegans), UNC13D
计算分子量 1090 aa, 103 kDa
观测分子量 120 kDa
GenBank蛋白编号BC067084
基因名称 UNC13D
Gene ID (NCBI) 201294
RRIDAB_2882488
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ70J99
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UNC13D antibody 67193-1-IgDownload protocol
IHC protocol for UNC13D antibody 67193-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols