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UNC13D Monoclonal antibody

UNC13D Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C5C1

Cat no : 67193-1-Ig

Print datasheet

Synonyms

FHL3, HLH3, HPLH3, Munc13 4, Protein unc 13 homolog D, unc 13 homolog D (C. elegans), UNC13D

验证数据展示

  • WB analysis using 67193-1-Ig

    WB analysis using 67193-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67193-1-Ig (UNC13D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat spleen using 67193-1-Ig

    IHC staining of rat spleen using 67193-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 67193-1-Ig (UNC13D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UNC13D antibody included
  • IHC staining of rat spleen using 67193-1-Ig

    IHC staining of rat spleen using 67193-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 67193-1-Ig (UNC13D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UNC13D antibody included

经过测试的应用

Positive WB detected inTHP-1 cells, RAW 264.7 cells, pig spleen tissue, rat spleen tissue, K-562 cells, Jurkat cells, HL-60 cells
Positive IHC detected inrat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy UNC13D Ready-To-Use IHC Kit. UNC13D primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67193-1-Ig targets UNC13D in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat, pig
Immunogen UNC13D fusion protein Ag10497 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name unc-13 homolog D (C. elegans)
Synonyms FHL3, HLH3, HPLH3, Munc13 4, Protein unc 13 homolog D, unc 13 homolog D (C. elegans), UNC13D
Calculated Molecular Weight 1090 aa, 103 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC067084
Gene Symbol UNC13D
Gene ID (NCBI) 201294
RRIDAB_2882488
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ70J99
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UNC13D antibody 67193-1-IgDownload protocol
IHC protocol for UNC13D antibody 67193-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols