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验证数据展示

  • WB analysis using CL488-67193

    WB analysis using CL488-67193

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-67193 (UNC13D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CoraLite® Plus 488-conjugated UNC13D Monoclonal antibody

UNC13D Monoclonal Antibody for WB
Cat No. CL488-67193

Print datasheet

CloneNo. 1C5C1

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB

Synonyms

FHL3, HLH3, HPLH3, Munc13 4, Protein unc 13 homolog D, unc 13 homolog D (C. elegans), UNC13D

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HL-60 cells
Planning an IHC experiment? We recommend our IHCeasy UNC13D Ready-To-Use IHC Kit. UNC13D primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67193-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67193 targets UNC13D in WB applications and shows reactivity with Human samples.

Tested Applications WB Application Description
Tested Reactivity Human
Immunogen UNC13D fusion protein Ag10497 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name unc-13 homolog D (C. elegans)
Synonyms FHL3, HLH3, HPLH3, Munc13 4, Protein unc 13 homolog D, unc 13 homolog D (C. elegans), UNC13D
Calculated Molecular Weight 1090 aa, 103 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC067084
Gene Symbol UNC13D
Gene ID (NCBI) 201294
RRIDAB_3084322
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ70J99
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UNC13D is a member of the UNC13 family, containing similar domain structure as other family members but lacking an N-terminal phorbol ester-binding C1 domain present in other Munc13 proteins. The protein appears to play a role in vesicle maturation during exocytosis and is involved in regulation of cytolytic granules secretion.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 UNC13D antibody CL488-67193Download protocol
Standard Protocols
Click here to view our Standard Protocols