验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HL-60 cells |
Planning an IHC experiment? We recommend our IHCeasy UNC13D Ready-To-Use IHC Kit. UNC13D primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67193-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67193 targets UNC13D in WB applications and shows reactivity with Human samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | Human |
| 免疫原 | UNC13D fusion protein Ag10497 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | unc-13 homolog D (C. elegans) |
| 别名 | FHL3, HLH3, HPLH3, Munc13 4, Protein unc 13 homolog D, unc 13 homolog D (C. elegans), UNC13D |
| 计算分子量 | 1090 aa, 103 kDa |
| 观测分子量 | 120 kDa |
| GenBank蛋白编号 | BC067084 |
| 基因名称 | UNC13D |
| Gene ID (NCBI) | 201294 |
| RRID | AB_3084322 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q70J99 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UNC13D is a member of the UNC13 family, containing similar domain structure as other family members but lacking an N-terminal phorbol ester-binding C1 domain present in other Munc13 proteins. The protein appears to play a role in vesicle maturation during exocytosis and is involved in regulation of cytolytic granules secretion.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 488 UNC13D antibody CL488-67193 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |