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CoraLite® Plus 488-conjugated UNG Polyclonal antibody

UNG Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12394

Print datasheet

Synonyms

Uracil-DNA glycosylase, UNG1, EC:3.2.2.27, DGU

验证数据展示

  • IF Staining of HeLa using CL488-12394

    IF Staining of HeLa using CL488-12394

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 UNG antibody (CL488-12394) at dilution of 1:200, CL594-Phalloidin (red).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy UNG Ready-To-Use IHC Kit. UNG primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 12394-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12394 targets UNG in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen UNG fusion protein Ag3072 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name uracil-DNA glycosylase
Synonyms Uracil-DNA glycosylase, UNG1, EC:3.2.2.27, DGU
Calculated Molecular Weight 304 aa, 34 kDa
Observed Molecular Weight 28 kDa, 34-40 kDa
GenBank Accession NumberBC015205
Gene Symbol UNG
Gene ID (NCBI) 7374
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13051
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UNG(Uracil-DNA glycosylase) removes uracil in DNA resulting from deamination of cytosine or replicative incorporation of dUMP instead of dTMP. Thus, UNG plays a role in suppressing GC-to-AT transition mutations.The UNG gene encodes 2 isoforms that are individually targeted to the mitochondria and the nucleus(PMID:12369930). Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 (HIGM5).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 UNG antibody CL488-12394Download protocol
Standard Protocols
Click here to view our Standard Protocols