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  • Featured Product
  • KD/KO Validated

UPF1 Polyclonal antibody, PBS Only

UPF1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28384-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 28384-1-AP (same clone as 28384-1-PBS)

    WB analysis using 28384-1-AP (same clone as 28384-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28384-1-AP (UPF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28384-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 28384-1-AP (same clone as 28384-1-PBS)

    WB analysis of HEK-293 using 28384-1-AP (same clone as 28384-1-PBS)

    WB result of UPF1 antibody (28384-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UPF1 transfected HEK-293 cells. This data was developed using the same antibody clone with 28384-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 28384-1-AP (same clone as 28384-1-PBS)

    IHC staining of human breast cancer using 28384-1-AP (same clone as 28384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28384-1-AP (UPF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28384-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 28384-1-AP (same clone as 28384-1-PBS)

    IHC staining of human breast cancer using 28384-1-AP (same clone as 28384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28384-1-AP (UPF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28384-1-PBS in a different storage buffer formulation.

产品信息

28384-1-PBS targets UPF1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen UPF1 fusion protein Ag29057 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name UPF1 regulator of nonsense transcripts homolog (yeast)
Synonyms
Calculated Molecular Weight123 kDa
Observed Molecular Weight 123-130 kDa
GenBank Accession NumberBC039817
Gene Symbol UPF1
Gene ID (NCBI) 5976
RRIDAB_2881130
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92900
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Up-Frameshift Suppressor 1 Homolog (UPF1) is the central factor in nonsense-mediated mRNA decay (NMD) and is also directly involved in telomere homeostasis, DNA replication, histone mRNA degradation and staufen-mediated mRNA decay (PMID: 29382845). It is a potential modulator of MALAT1 and that UPF1/MALAT1 pathway could be a therapeutic target for gastric cancer (PMID: 28942451). The molecular mass of UPF1 is 123-130 kDa.