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UQCRC2 Recombinant antibody, PBS Only

UQCRC2 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240454D9

Cat no : 83667-2-PBS

Print datasheet

Synonyms

Ubiquinol-cytochrome-c reductase complex core protein 2, QCR2, Cytochrome b-c1 complex subunit 2, mitochondrial, Core protein II, Complex III subunit 2

验证数据展示

  • WB analysis using 83667-2-RR (same clone as 83667-2-PBS)

    WB analysis using 83667-2-RR (same clone as 83667-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83667-2-RR (UQCRC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83667-2-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 83667-2-RR (same clone as 83667-2-PBS)

    IHC staining of human stomach cancer using 83667-2-RR (same clone as 83667-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83667-2-RR (UQCRC2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83667-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UQCRC2 antibody included
  • FC experiment of HepG2 using 83667-2-RR (same clone as 83667-2-PBS)

    FC experiment of HepG2 using 83667-2-RR (same clone as 83667-2-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug UQCRC2 Recombinant antibody (83667-2-RR, Clone:240454D9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83667-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83667-2-RR

    Affinity and Kinetic Characterization of 83667-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83667-2-RR against Human UQCRC2 were performed. The affinity constant is 21.8 nM.

Planning an IHC experiment? We recommend our IHCeasy UQCRC2 Ready-To-Use IHC Kit. UQCRC2 primary antibody included.

产品信息

83667-2-PBS targets UQCRC2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen UQCRC2 fusion protein Ag6432 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ubiquinol-cytochrome c reductase core protein II
Synonyms Ubiquinol-cytochrome-c reductase complex core protein 2, QCR2, Cytochrome b-c1 complex subunit 2, mitochondrial, Core protein II, Complex III subunit 2
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC000484
Gene Symbol UQCRC2
Gene ID (NCBI) 7385
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP22695
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UQCRC2(Ubiquinol-cytochrome-c reductase complex core protein 2) is also named as complex III subunit 2,core protein II and belongs to the UQCRC2/QCR2 subfamily. The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1) and is part of the mitochondrial respiratory chain. This protein distributes in mitochondrion inner membrane, peripheral membrane protein and matrix side.