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验证数据展示

  • WB analysis using 83667-2-RR

    WB analysis using 83667-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83667-2-RR (UQCRC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 83667-2-RR

    IHC staining of human stomach cancer using 83667-2-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83667-2-RR (UQCRC2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

    Ready-to-use IHC kits available with UQCRC2 antibody included
  • FC experiment of HepG2 using 83667-2-RR

    FC experiment of HepG2 using 83667-2-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug UQCRC2 Recombinant antibody (83667-2-RR, Clone:240454D9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83667-2-RR

    Affinity and Kinetic Characterization of 83667-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83667-2-RR against Human UQCRC2 were performed. The affinity constant is 21.8 nM.

UQCRC2 Recombinant antibody

UQCRC2 Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83667-2-RR

产品说明书

CloneNo. 240454D9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), ELISA

别名

Ubiquinol-cytochrome-c reductase complex core protein 2, QCR2, Cytochrome b-c1 complex subunit 2, mitochondrial, Core protein II, Complex III subunit 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HEK-293 cells, Jurkat cells, HeLa cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy UQCRC2 Ready-To-Use IHC Kit. UQCRC2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83667-2-RR targets UQCRC2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 UQCRC2 fusion protein Ag6432 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ubiquinol-cytochrome c reductase core protein II
别名 Ubiquinol-cytochrome-c reductase complex core protein 2, QCR2, Cytochrome b-c1 complex subunit 2, mitochondrial, Core protein II, Complex III subunit 2
计算分子量 48 kDa
观测分子量 48 kDa
GenBank蛋白编号BC000484
基因名称 UQCRC2
Gene ID (NCBI) 7385
RRIDAB_3671273
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP22695
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UQCRC2(Ubiquinol-cytochrome-c reductase complex core protein 2) is also named as complex III subunit 2,core protein II and belongs to the UQCRC2/QCR2 subfamily. The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1) and is part of the mitochondrial respiratory chain. This protein distributes in mitochondrion inner membrane, peripheral membrane protein and matrix side.

实验方案

Product Specific Protocols
WB protocol for UQCRC2 antibody 83667-2-RRDownload protocol
IHC protocol for UQCRC2 antibody 83667-2-RRDownload protocol
FC protocol for UQCRC2 antibody 83667-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols