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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated UQCRC2 Polyclonal antibody

UQCRC2 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-14742

Print datasheet

Synonyms

UQCR2, Ubiquinol-cytochrome-c reductase complex core protein 2, QCR2, Cytochrome b-c1 complex subunit 2, mitochondrial, Core protein II

验证数据展示

  • IF Staining of HepG2 using CL647-14742

    IF Staining of HepG2 using CL647-14742

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 647 UQCRC2 antibody (CL647-14742) at dilution of 1:200.

  • FC experiment of HepG2 using CL647-14742

    FC experiment of HepG2 using CL647-14742

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human UQCRC2 (CL647-14742) (red), or 0.8 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy UQCRC2 Ready-To-Use IHC Kit. UQCRC2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14742-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-14742 targets UQCRC2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen UQCRC2 fusion protein Ag6432 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquinol-cytochrome c reductase core protein II
Synonyms UQCR2, Ubiquinol-cytochrome-c reductase complex core protein 2, QCR2, Cytochrome b-c1 complex subunit 2, mitochondrial, Core protein II
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC000484
Gene Symbol UQCRC2
Gene ID (NCBI) 7385
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22695
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UQCRC2(Ubiquinol-cytochrome-c reductase complex core protein 2) is also named as complex III subunit 2,core protein II and belongs to the UQCRC2/QCR2 subfamily. The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1) and is part of the mitochondrial respiratory chain. This protein distributes in mitochondrion inner membrane, peripheral membrane protein and matrix side.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 UQCRC2 antibody CL647-14742Download protocol
FC protocol for CL Plus 647 UQCRC2 antibody CL647-14742Download protocol
Standard Protocols
Click here to view our Standard Protocols