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  • Featured Product
  • KD/KO Validated

URG4 Polyclonal antibody

URG4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11998-1-AP

Print datasheet

Synonyms

URGCP, Up-regulator of cell proliferation, up regulated gene 4, KIAA1507, HBxAg up-regulated gene 4 protein

验证数据展示

  • WB analysis of HEK-293 using 11998-1-AP

    WB analysis of HEK-293 using 11998-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11998-1-AP (URG4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 11998-1-AP

    IP experiment of HEK-293 using 11998-1-AP

    IP result of anti-URG4 (IP:11998-1-AP, 4ug; Detection:11998-1-AP 1:300) with HEK-293 cells lysate 880ug.

  • IHC staining of human stomach cancer using 11998-1-AP

    IHC staining of human stomach cancer using 11998-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11998-1-AP (URG4 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HEK-293 using 11998-1-AP

    WB analysis of HEK-293 using 11998-1-AP

    WB result of URG4 antibody (11998-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-URG4 transfected HEK-293 cells.

  • IF Staining of HeLa using 11998-1-AP

    IF Staining of HeLa using 11998-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using URG4 antibody (11998-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman stomach cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11998-1-AP targets URG4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen URG4 fusion protein Ag2630 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name up-regulated gene 4
Synonyms URGCP, Up-regulator of cell proliferation, up regulated gene 4, KIAA1507, HBxAg up-regulated gene 4 protein
Calculated Molecular Weight 922 aa, 104 kDa
Observed Molecular Weight 104 kDa
GenBank Accession NumberBC018426
Gene Symbol URG4
Gene ID (NCBI) 55665
RRIDAB_1072845
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TCY9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

URG4 (upregulated gene 4), also known as upregulator of cell proliferation (URGCP), is an oncogene which is involved in the development and progression of various tumors. URG4 is upregulated in hepatocellular carcinoma (HCC), ovarian cancer, and other types of cancer. Overexpression of URG4 not only results in tumour progression but also in metastasis and recurrence.

实验方案

Product Specific Protocols
WB protocol for URG4 antibody 11998-1-APDownload protocol
IHC protocol for URG4 antibody 11998-1-APDownload protocol
IF protocol for URG4 antibody 11998-1-APDownload protocol
IP protocol for URG4 antibody 11998-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Bone

Osteoblast-derived exosomes promote osteogenic differentiation of osteosarcoma cells via URG4/Wnt signaling pathway

Authors - Yuanxi Leng
View Article
humanIHC,WB

J Orthop Surg Res

URG4 mediates cell proliferation and cell cycle in osteosarcoma via GSK3β/β-catenin/cyclin D1 signaling pathway.

Authors - Yayun Liu
  • KD Validated
View Article