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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated URG4 Polyclonal antibody

URG4 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11998

Print datasheet

Synonyms

URGCP, Up-regulator of cell proliferation, up regulated gene 4, KIAA1507, HBxAg up-regulated gene 4 protein

验证数据展示

  • IF Staining of HeLa using CL488-11998

    IF Staining of HeLa using CL488-11998

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 URG4 antibody (CL488-11998) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HEK-293 using CL488-11998

    FC experiment of HEK-293 using CL488-11998

    1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Urg4 Polyclonal Antibody (CL488-11998)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 11998-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11998 targets URG4 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen URG4 fusion protein Ag2630 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name up-regulated gene 4
Synonyms URGCP, Up-regulator of cell proliferation, up regulated gene 4, KIAA1507, HBxAg up-regulated gene 4 protein
Calculated Molecular Weight 922 aa, 104 kDa
Observed Molecular Weight 104 kDa
GenBank Accession NumberBC018426
Gene Symbol URG4
Gene ID (NCBI) 55665
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TCY9
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

URG4 (upregulated gene 4), also known as upregulator of cell proliferation (URGCP), is an oncogene which is involved in the development and progression of various tumors. URG4 is upregulated in hepatocellular carcinoma (HCC), ovarian cancer, and other types of cancer. Overexpression of URG4 not only results in tumour progression but also in metastasis and recurrence.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 URG4 antibody CL488-11998Download protocol
FC protocol for CL Plus 488 URG4 antibody CL488-11998Download protocol
Standard Protocols
Click here to view our Standard Protocols