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验证数据展示

  • IF Staining of HeLa using CL488-11998

    IF Staining of HeLa using CL488-11998

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 URG4 antibody (CL488-11998) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HEK-293 using CL488-11998

    FC experiment of HEK-293 using CL488-11998

    1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Urg4 Polyclonal Antibody (CL488-11998)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated URG4 Polyclonal antibody

URG4 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-11998

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

URGCP, Up-regulator of cell proliferation, up regulated gene 4, KIAA1507, HBxAg up-regulated gene 4 protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy URG4 Ready-To-Use IHC Kit. URG4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11998-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11998 targets URG4 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 URG4 fusion protein Ag2630 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 up-regulated gene 4
别名 URGCP, Up-regulator of cell proliferation, up regulated gene 4, KIAA1507, HBxAg up-regulated gene 4 protein
计算分子量 922 aa, 104 kDa
观测分子量 104 kDa
GenBank蛋白编号BC018426
基因名称 URG4
Gene ID (NCBI) 55665
RRIDAB_3672524
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TCY9
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

URG4 (upregulated gene 4), also known as upregulator of cell proliferation (URGCP), is an oncogene which is involved in the development and progression of various tumors. URG4 is upregulated in hepatocellular carcinoma (HCC), ovarian cancer, and other types of cancer. Overexpression of URG4 not only results in tumour progression but also in metastasis and recurrence.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 URG4 antibody CL488-11998Download protocol
FC protocol for CL Plus 488 URG4 antibody CL488-11998Download protocol
Standard Protocols
Click here to view our Standard Protocols