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验证数据展示

  • WB analysis using 67114-1-Ig

    WB analysis using 67114-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

USF1 Monoclonal antibody

USF1 Monoclonal Antibody for WB, ELISA
Cat No. 67114-1-Ig

Print datasheet

CloneNo. 1C8F1

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Synonyms

bHLHb11, FCHL, FCHL1, HYPLIP1, MLTF, MLTFI, UEF, Upstream stimulatory factor 1, USF, USF1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HT-29 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, Jurkat cells, PC-12 cells, 4T1 cells
Planning an IHC experiment? We recommend our IHCeasy USF1 Ready-To-Use IHC Kit. USF1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67114-1-Ig targets USF1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen USF1 fusion protein Ag17785 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name upstream transcription factor 1
Synonyms bHLHb11, FCHL, FCHL1, HYPLIP1, MLTF, MLTFI, UEF, Upstream stimulatory factor 1, USF, USF1
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 38-43 kDa
GenBank Accession NumberBC035505
Gene Symbol USF1
Gene ID (NCBI) 7391
RRIDAB_2882418
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP22415
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Upstream stimulatory factor 1 (USF1),also name Class B basic helix-loop-helix protein 11. it is a major late transcription factor 1.USF1 is a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E box motifs. USF1 has been linked to familial combined hyperlipidemia (FCHL). The USF1 exists two isoform and the calculated molecular weight of USF1 is a 32 kDa and 27 kDa. USF1 has acetylated form and phosphorylated form, so the molecular weight of USF1 can migrate to about 40 kDa

实验方案

Product Specific Protocols
WB protocol for USF1 antibody 67114-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols