验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, HT-29 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, Jurkat cells, PC-12 cells, 4T1 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67114-1-Ig targets USF1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | Human, mouse, rat |
免疫原 | USF1 fusion protein Ag17785 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | upstream transcription factor 1 |
别名 | bHLHb11, FCHL, FCHL1, HYPLIP1, MLTF, MLTFI, UEF, Upstream stimulatory factor 1, USF, USF1 |
计算分子量 | 34 kDa |
观测分子量 | 38-43 kDa |
GenBank蛋白编号 | BC035505 |
基因名称 | USF1 |
Gene ID (NCBI) | 7391 |
RRID | AB_2882418 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | P22415 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Upstream stimulatory factor 1 (USF1),also name Class B basic helix-loop-helix protein 11. it is a major late transcription factor 1.USF1 is a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E box motifs. USF1 has been linked to familial combined hyperlipidemia (FCHL). The USF1 exists two isoform and the calculated molecular weight of USF1 is a 32 kDa and 27 kDa. USF1 has acetylated form and phosphorylated form, so the molecular weight of USF1 can migrate to about 40 kDa
实验方案
Product Specific Protocols | |
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WB protocol for USF1 antibody 67114-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |