USF1 Monoclonal antibody
USF1 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1C8F1
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, HT-29 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, Jurkat cells, PC-12 cells, 4T1 cells |
Planning an IHC experiment? We recommend our IHCeasy USF1 Ready-To-Use IHC Kit. USF1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67114-1-Ig targets USF1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | Human, mouse, rat |
| Immunogen | USF1 fusion protein Ag17785 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | upstream transcription factor 1 |
| Synonyms | bHLHb11, FCHL, FCHL1, HYPLIP1, MLTF, MLTFI, UEF, Upstream stimulatory factor 1, USF, USF1 |
| Calculated Molecular Weight | 34 kDa |
| Observed Molecular Weight | 38-43 kDa |
| GenBank Accession Number | BC035505 |
| Gene Symbol | USF1 |
| Gene ID (NCBI) | 7391 |
| RRID | AB_2882418 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P22415 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Upstream stimulatory factor 1 (USF1),also name Class B basic helix-loop-helix protein 11. it is a major late transcription factor 1.USF1 is a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E box motifs. USF1 has been linked to familial combined hyperlipidemia (FCHL). The USF1 exists two isoform and the calculated molecular weight of USF1 is a 32 kDa and 27 kDa.
USF1 has acetylated form and phosphorylated form, so the molecular weight of USF1 can migrate to about 40 kDa
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for USF1 antibody 67114-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
