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验证数据展示

  • FC experiment of MCF-7 using CL488-67114

    FC experiment of MCF-7 using CL488-67114

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human USF1 (CL488-67114, Clone:1C8F1) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated USF1 Monoclonal antibody

USF1 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67114

产品说明书

CloneNo. 1C8F1

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

FC (Intra)

别名

HYPLIP1, FCHL1, FCHL, Class B basic helix-loop-helix protein 11, bHLHb11

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy USF1 Ready-To-Use IHC Kit. USF1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67114-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67114 targets USF1 in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, mouse, rat
免疫原 USF1 fusion protein Ag17785 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 upstream transcription factor 1
别名 HYPLIP1, FCHL1, FCHL, Class B basic helix-loop-helix protein 11, bHLHb11
计算分子量 34 kDa
观测分子量 38-43 kDa
GenBank蛋白编号BC035505
基因名称 USF1
Gene ID (NCBI) 7391
RRIDAB_3084309
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP22415
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Upstream stimulatory factor 1 (USF1),also name Class B basic helix-loop-helix protein 11. it is a major late transcription factor 1.USF1 is a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E box motifs. USF1 has been linked to familial combined hyperlipidemia (FCHL). The USF1 exists two isoform and the calculated molecular weight of USF1 is a 32 kDa and 27 kDa. USF1 has acetylated form and phosphorylated form, so the molecular weight of USF1 can migrate to about 40 kDa

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 USF1 antibody CL488-67114Download protocol
Standard Protocols
Click here to view our Standard Protocols