验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67114 targets USF1 in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 | USF1 fusion protein Ag17785 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | upstream transcription factor 1 |
| 别名 | HYPLIP1, FCHL1, FCHL, Class B basic helix-loop-helix protein 11, bHLHb11 |
| 计算分子量 | 34 kDa |
| 观测分子量 | 38-43 kDa |
| GenBank蛋白编号 | BC035505 |
| 基因名称 | USF1 |
| Gene ID (NCBI) | 7391 |
| RRID | AB_3084309 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P22415 |
| 储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Upstream stimulatory factor 1 (USF1),also name Class B basic helix-loop-helix protein 11. it is a major late transcription factor 1.USF1 is a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E box motifs. USF1 has been linked to familial combined hyperlipidemia (FCHL). The USF1 exists two isoform and the calculated molecular weight of USF1 is a 32 kDa and 27 kDa. USF1 has acetylated form and phosphorylated form, so the molecular weight of USF1 can migrate to about 40 kDa
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 USF1 antibody CL488-67114 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |