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CoraLite® Plus 488-conjugated USF1 Monoclonal antibody

USF1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C8F1

Cat no : CL488-67114

Print datasheet

Synonyms

HYPLIP1, FCHL1, FCHL, Class B basic helix-loop-helix protein 11, bHLHb11

验证数据展示

  • FC experiment of MCF-7 using CL488-67114

    FC experiment of MCF-7 using CL488-67114

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human USF1 (CL488-67114, Clone:1C8F1) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy USF1 Ready-To-Use IHC Kit. USF1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67114-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67114 targets USF1 in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human, mouse, rat
Immunogen USF1 fusion protein Ag17785 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name upstream transcription factor 1
Synonyms HYPLIP1, FCHL1, FCHL, Class B basic helix-loop-helix protein 11, bHLHb11
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 38-43 kDa
GenBank Accession NumberBC035505
Gene Symbol USF1
Gene ID (NCBI) 7391
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP22415
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Upstream stimulatory factor 1 (USF1),also name Class B basic helix-loop-helix protein 11. it is a major late transcription factor 1.USF1 is a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E box motifs. USF1 has been linked to familial combined hyperlipidemia (FCHL). The USF1 exists two isoform and the calculated molecular weight of USF1 is a 32 kDa and 27 kDa. USF1 has acetylated form and phosphorylated form, so the molecular weight of USF1 can migrate to about 40 kDa

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 USF1 antibody CL488-67114Download protocol
Standard Protocols
Click here to view our Standard Protocols