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  • Featured Product
  • KD/KO Validated

USP1 Monoclonal antibody

USP1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C10H6

Cat no : 66069-1-Ig

Print datasheet

Synonyms

Deubiquitinating enzyme 1, hUBP, ubiquitin specific peptidase 1, Ubiquitin thiolesterase 1, UBP, USP1

验证数据展示

  • WB analysis of HepG2 using 66069-1-Ig

    WB analysis of HepG2 using 66069-1-Ig

    WB result of USP1 antibody (66069-1-Ig, 1:2000) with si-Control and si-USP1 transfected HepG2 & HEK293 cells.

  • WB analysis of HepG2 using 66069-1-Ig

    WB analysis of HepG2 using 66069-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66069-1-Ig (USP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 66069-1-Ig

    IHC staining of human cervical cancer using 66069-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66069-1-Ig (USP1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cervical cancer using 66069-1-Ig

    IHC staining of human cervical cancer using 66069-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66069-1-Ig (USP1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66069-1-Ig targets USP1 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, rat
Cited Reactivityhuman, monkey
Immunogen USP1 fusion protein Ag5904 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ubiquitin specific peptidase 1
Synonyms Deubiquitinating enzyme 1, hUBP, ubiquitin specific peptidase 1, Ubiquitin thiolesterase 1, UBP, USP1
Calculated Molecular Weight 88 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC050525
Gene Symbol USP1
Gene ID (NCBI) 7398
RRIDAB_11043340
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO94782
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP1(Ubiquitin carboxyl-terminal hydrolase 1) is a negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2.It is Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA.This protein, which consists of 785 amino acids with a deduced molecular mass of 88.2 kDa, possesses His and Cys domains that are highly conserved in all members of the ubiquitin-specific processing (UBP) family of proteases(PMID:9806842). The USP1 enzyme can undergo autocleavage, resulting in a 100 kDa N-terminal fragment and a 14 kDa C-terminal fragment(PMID:18082604).

实验方案

Product Specific Protocols
WB protocol for USP1 antibody 66069-1-IgDownload protocol
IHC protocol for USP1 antibody 66069-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,monkeyWB

J Virol

Ubiquitin-specific proteinase 1 stabilizes PRRSV nonstructural protein Nsp1β to promote viral replication by regulating K48 ubiquitination

Authors - Yunyun Zhai
  • KD Validated
View Article