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验证数据展示

  • WB analysis of HepG2 using 66069-1-Ig (same clone as 66069-1-PBS)

    WB analysis of HepG2 using 66069-1-Ig (same clone as 66069-1-PBS)

    WB result of USP1 antibody (66069-1-Ig, 1:2000) with si-Control and si-USP1 transfected HepG2 & HEK293 cells. This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66069-1-Ig (same clone as 66069-1-PBS)

    WB analysis of HepG2 using 66069-1-Ig (same clone as 66069-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66069-1-Ig (USP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 66069-1-Ig (same clone as 66069-1-PBS)

    IHC staining of human cervical cancer using 66069-1-Ig (same clone as 66069-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66069-1-Ig (USP1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 66069-1-Ig (same clone as 66069-1-PBS)

    IHC staining of human cervical cancer using 66069-1-Ig (same clone as 66069-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66069-1-Ig (USP1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 66069-1-Ig (same clone as 66069-1-PBS)

    WB analysis of NIH/3T3 using 66069-1-Ig (same clone as 66069-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66069-1-Ig (USP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 66069-1-Ig (same clone as 66069-1-PBS)

    WB analysis of HSC-T6 using 66069-1-Ig (same clone as 66069-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66069-1-Ig (USP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

USP1 Monoclonal antibody, PBS Only

USP1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66069-1-PBS

产品说明书

CloneNo. 1C10H6

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

1C10H6, Deubiquitinating enzyme 1, EC:3.4.19.12, hUBP, Ubiquitin carboxyl-terminal hydrolase 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66069-1-PBS targets USP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 USP1 fusion protein Ag5904 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 1
别名 1C10H6, Deubiquitinating enzyme 1, EC:3.4.19.12, hUBP, Ubiquitin carboxyl-terminal hydrolase 1
计算分子量 88 kDa
观测分子量 100 kDa
GenBank蛋白编号BC050525
基因名称 USP1
Gene ID (NCBI) 7398
RRIDAB_11043340
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO94782
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

USP1(Ubiquitin carboxyl-terminal hydrolase 1) is a negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2.It is Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA.This protein, which consists of 785 amino acids with a deduced molecular mass of 88.2 kDa, possesses His and Cys domains that are highly conserved in all members of the ubiquitin-specific processing (UBP) family of proteases(PMID:9806842). The USP1 enzyme can undergo autocleavage, resulting in a 100 kDa N-terminal fragment and a 14 kDa C-terminal fragment(PMID:18082604).