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  • Featured Product
  • KD/KO Validated

USP1 Monoclonal antibody, PBS Only

USP1 Monoclonal Antibody for WB,IHC,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat

Applications

WB,IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1C10H6

Cat No : 66069-1-PBS

Print datasheet

Synonyms

Deubiquitinating enzyme 1, hUBP, ubiquitin specific peptidase 1, Ubiquitin thiolesterase 1, UBP, USP1

验证数据展示

  • WB analysis of HepG2 using 66069-1-Ig (same clone as 66069-1-PBS)

    WB analysis of HepG2 using 66069-1-Ig (same clone as 66069-1-PBS)

    WB result of USP1 antibody (66069-1-Ig, 1:2000) with si-Control and si-USP1 transfected HepG2 & HEK293 cells. This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66069-1-Ig (same clone as 66069-1-PBS)

    WB analysis of HepG2 using 66069-1-Ig (same clone as 66069-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66069-1-Ig (USP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 66069-1-Ig (same clone as 66069-1-PBS)

    IHC staining of human cervical cancer using 66069-1-Ig (same clone as 66069-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66069-1-Ig (USP1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 66069-1-Ig (same clone as 66069-1-PBS)

    IHC staining of human cervical cancer using 66069-1-Ig (same clone as 66069-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66069-1-Ig (USP1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

产品信息

66069-1-PBS targets USP1 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB,IHC,ELISA Application Description
Tested Reactivity human, rat
Immunogen USP1 fusion protein Ag5904 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ubiquitin specific peptidase 1
Synonyms Deubiquitinating enzyme 1, hUBP, ubiquitin specific peptidase 1, Ubiquitin thiolesterase 1, UBP, USP1
Calculated Molecular Weight 88 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC050525
Gene Symbol USP1
Gene ID (NCBI) 7398
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO94782
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

USP1(Ubiquitin carboxyl-terminal hydrolase 1) is a negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2.It is Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA.This protein, which consists of 785 amino acids with a deduced molecular mass of 88.2 kDa, possesses His and Cys domains that are highly conserved in all members of the ubiquitin-specific processing (UBP) family of proteases(PMID:9806842). The USP1 enzyme can undergo autocleavage, resulting in a 100 kDa N-terminal fragment and a 14 kDa C-terminal fragment(PMID:18082604).