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  • Featured Product
  • KD/KO Validated

USP10 Monoclonal antibody

USP10 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B10C9

Cat no : 67917-1-Ig

Print datasheet

Synonyms

Deubiquitinating enzyme 10, KIAA0190, Ubiquitin thiolesterase 10, UBPO, USP10

验证数据展示

  • WB analysis using 67917-1-Ig

    WB analysis using 67917-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67917-1-Ig (USP10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IHC staining of human liver cancer using 67917-1-Ig

    IHC staining of human liver cancer using 67917-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67917-1-Ig (USP10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 67917-1-Ig

    IF Staining of HEK-293 using 67917-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using USP10 antibody (67917-1-Ig, Clone: 1B10C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis of MCF-7 using 67917-1-Ig

    WB analysis of MCF-7 using 67917-1-Ig

    WB result of USP10 antibody (67917-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP10 transfected MCF-7 cells.

经过测试的应用

Positive WB detected inLNCaP cells, MCF-7 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67917-1-Ig targets USP10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Immunogen USP10 fusion protein Ag7233 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ubiquitin specific peptidase 10
Synonyms Deubiquitinating enzyme 10, KIAA0190, Ubiquitin thiolesterase 10, UBPO, USP10
Calculated Molecular Weight 87 kDa, 92 kDa
Observed Molecular Weight100-110 kDa
GenBank Accession NumberBC000263
Gene Symbol USP10
Gene ID (NCBI) 9100
RRIDAB_2918671
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14694
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP10, a cytoplasmic ubiquitin-specific protease, deubiquitinates p53, reversing Mdm2-induced p53 nuclear export and degradation. After DNA damage, USP10 is stabilized, and a fraction of USP10 translocates to the nucleus to activate p53. The translocation and stabilization of USP10 is regulated by ATM -mediated phosphorylation of USP10 at Thr42 and Ser337. (PMID: 20096447, PMID: 26876938)

实验方案

Product Specific Protocols
WB protocol for USP10 antibody 67917-1-IgDownload protocol
IHC protocol for USP10 antibody 67917-1-IgDownload protocol
IF protocol for USP10 antibody 67917-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Rep

hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility

Authors - Xiaoli Wang
  • KD Validated
View Article