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USP10 Monoclonal antibody, PBS Only

USP10 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B10C9

Cat no : 67917-1-PBS

Print datasheet

Synonyms

Deubiquitinating enzyme 10, KIAA0190, Ubiquitin thiolesterase 10, UBPO, USP10

验证数据展示

  • WB analysis using 67917-1-Ig (same clone as 67917-1-PBS)

    WB analysis using 67917-1-Ig (same clone as 67917-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67917-1-Ig (USP10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67917-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67917-1-Ig (same clone as 67917-1-PBS)

    IHC staining of human liver cancer using 67917-1-Ig (same clone as 67917-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67917-1-Ig (USP10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67917-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 67917-1-Ig (same clone as 67917-1-PBS)

    IF Staining of HEK-293 using 67917-1-Ig (same clone as 67917-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using USP10 antibody (67917-1-Ig, Clone: 1B10C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67917-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 67917-1-Ig (same clone as 67917-1-PBS)

    WB analysis of MCF-7 using 67917-1-Ig (same clone as 67917-1-PBS)

    WB result of USP10 antibody (67917-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP10 transfected MCF-7 cells. This data was developed using the same antibody clone with 67917-1-PBS in a different storage buffer formulation.

产品信息

67917-1-PBS targets USP10 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen USP10 fusion protein Ag7233 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ubiquitin specific peptidase 10
Synonyms Deubiquitinating enzyme 10, KIAA0190, Ubiquitin thiolesterase 10, UBPO, USP10
Calculated Molecular Weight 87 kDa, 92 kDa
Observed Molecular Weight100-110 kDa
GenBank Accession NumberBC000263
Gene Symbol USP10
Gene ID (NCBI) 9100
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14694
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

USP10, a cytoplasmic ubiquitin-specific protease, deubiquitinates p53, reversing Mdm2-induced p53 nuclear export and degradation. After DNA damage, USP10 is stabilized, and a fraction of USP10 translocates to the nucleus to activate p53. The translocation and stabilization of USP10 is regulated by ATM -mediated phosphorylation of USP10 at Thr42 and Ser337. (PMID: 20096447, PMID: 26876938)