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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated USP13 Monoclonal antibody

USP13 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

6E8C10

Cat no : CL594-66176

Print datasheet

Synonyms

Ubiquitin carboxyl-terminal hydrolase 13, ISOT-3, ISOT3, Isopeptidase T-3, EC:3.4.19.12

验证数据展示

  • IF Staining of HepG2 using CL594-66176

    IF Staining of HepG2 using CL594-66176

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594-conjugated USP13 antibody (CL594-66176, Clone: 6E8C10 ) at dilution of 1:100.

  • IF Staining of HepG2 using CL594-66176

    IF Staining of HepG2 using CL594-66176

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 USP13 antibody (CL594-66176, Clone: 6E8C10 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66176-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66176 targets USP13 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen USP13 fusion protein Ag10506 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ubiquitin specific peptidase 13 (isopeptidase T-3)
Synonyms Ubiquitin carboxyl-terminal hydrolase 13, ISOT-3, ISOT3, Isopeptidase T-3, EC:3.4.19.12
Calculated Molecular Weight 863 aa, 97 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC016146
Gene Symbol USP13
Gene ID (NCBI) 8975
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ92995
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP13(Ubiquitin carboxyl-terminal hydrolase 13) is also named as ISOT3 and belongs to the peptidase C19 family. It is involved in ubiquitin-dependent protein catabolism. USP13 is responsible for MITF deubiquitination, utilizing a short hairpin RNA library against known deubiquitinating enzymes. This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL594 USP13 antibody CL594-66176Download protocol
Standard Protocols
Click here to view our Standard Protocols