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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 82128-1-RR (same clone as 82128-1-PBS)

    WB analysis using 82128-1-RR (same clone as 82128-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82128-1-RR (USP14 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82128-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 82128-1-RR (same clone as 82128-1-PBS)

    WB analysis of HeLa using 82128-1-RR (same clone as 82128-1-PBS)

    WB result of USP14 antibody (82128-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP14 transfected HeLa cells. This data was developed using the same antibody clone with 82128-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse liver using 82128-1-RR (same clone as 82128-1-PBS)

    IP experiment of mouse liver using 82128-1-RR (same clone as 82128-1-PBS)

    IP result of anti-USP14 (IP:82128-1-RR, 4ug; Detection:82128-1-RR 1:2000) with mouse liver tissue lysate 1840 ug. This data was developed using the same antibody clone with 82128-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82128-1-PBS

    Affinity and Kinetic Characterization of 82128-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 82128-1-RR against Human USP14 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

USP14 Recombinant antibody, PBS Only

USP14 Uni-rAbTM Recombinant Antibody for WB, IP, Indirect ELISA
Cat No. 82128-1-PBS
CloneNo. 3G12

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

Ubiquitin thioesterase 14, Ubiquitin carboxyl-terminal hydrolase 14, TGT, EC:3.4.19.12, Deubiquitinating enzyme 14

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

82128-1-PBS targets USP14 in WB, IP, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IP, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen USP14 fusion protein Ag5979 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
Synonyms Ubiquitin thioesterase 14, Ubiquitin carboxyl-terminal hydrolase 14, TGT, EC:3.4.19.12, Deubiquitinating enzyme 14
Calculated Molecular Weight 54 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC003556
Gene Symbol USP14
Gene ID (NCBI) 9097
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP54578
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.