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验证数据展示

  • WB analysis using 82128-1-RR (same clone as 82128-1-PBS)

    WB analysis using 82128-1-RR (same clone as 82128-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82128-1-RR (USP14 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82128-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 82128-1-RR (same clone as 82128-1-PBS)

    WB analysis of HeLa using 82128-1-RR (same clone as 82128-1-PBS)

    WB result of USP14 antibody (82128-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP14 transfected HeLa cells. This data was developed using the same antibody clone with 82128-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse liver using 82128-1-RR (same clone as 82128-1-PBS)

    IP experiment of mouse liver using 82128-1-RR (same clone as 82128-1-PBS)

    IP result of anti-USP14 (IP:82128-1-RR, 4ug; Detection:82128-1-RR 1:2000) with mouse liver tissue lysate 1840 ug. This data was developed using the same antibody clone with 82128-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82128-1-PBS

    Affinity and Kinetic Characterization of 82128-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 82128-1-RR against Human USP14 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

USP14 Recombinant antibody, PBS Only

USP14 Uni-rAbTM Recombinant Antibody for WB, IP, Indirect ELISA
Cat No. 82128-1-PBS

产品说明书

CloneNo. 3G12

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IP, Indirect ELISA

别名

Ubiquitin thioesterase 14, Ubiquitin carboxyl-terminal hydrolase 14, TGT, EC:3.4.19.12, Deubiquitinating enzyme 14

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82128-1-PBS targets USP14 in WB, IP, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 USP14 fusion protein Ag5979 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
别名 Ubiquitin thioesterase 14, Ubiquitin carboxyl-terminal hydrolase 14, TGT, EC:3.4.19.12, Deubiquitinating enzyme 14
计算分子量 54 kDa
观测分子量60 kDa
GenBank蛋白编号BC003556
基因名称 USP14
Gene ID (NCBI) 9097
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP54578
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.