验证数据展示
经过测试的应用
Positive WB detected in | HCT 116 cells, HeLa cells, HEK-293T cells, DU 145 cells, Jurkat cells, NIH/3T3 cells, C6 cells |
Positive IP detected in | mouse liver tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82128-1-RR targets USP14 in WB, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | WB, IP, ELISA Application Description |
Tested Reactivity | Human, mouse, rat |
Immunogen | USP14 fusion protein Ag5979 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) |
Synonyms | Ubiquitin thioesterase 14, Ubiquitin carboxyl-terminal hydrolase 14, TGT, EC:3.4.19.12, Deubiquitinating enzyme 14 |
Calculated Molecular Weight | 54 kDa |
Observed Molecular Weight | 60 kDa |
GenBank Accession Number | BC003556 |
Gene Symbol | USP14 |
Gene ID (NCBI) | 9097 |
RRID | AB_3086466 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P54578 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for USP14 antibody 82128-1-RR | Download protocol |
IP protocol for USP14 antibody 82128-1-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |