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验证数据展示

  • WB analysis using CL750-82128

    WB analysis using CL750-82128

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-82128 (USP14 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis using CL750-82128

    WB analysis using CL750-82128

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-82128 (USP14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using CL750-82128

    FC experiment of HepG2 using CL750-82128

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated USP14 Recombinant antibody (CL750-82128, Clone:3G12)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 750-conjugated USP14 Recombinant antibody

USP14 Uni-rAbTM Recombinant Antibody for WB, FC (Intra)
Cat No. CL750-82128

产品说明书

CloneNo. 3G12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra)

别名

Ubiquitin thioesterase 14, Ubiquitin carboxyl-terminal hydrolase 14, TGT, EC:3.4.19.12, Deubiquitinating enzyme 14

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 750
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells, C6 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 82128-1-PBS

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-82128 targets USP14 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 USP14 fusion protein Ag5979 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
别名 Ubiquitin thioesterase 14, Ubiquitin carboxyl-terminal hydrolase 14, TGT, EC:3.4.19.12, Deubiquitinating enzyme 14
计算分子量 54 kDa
观测分子量60 kDa
GenBank蛋白编号BC003556
基因名称 USP14
Gene ID (NCBI) 9097
RRIDAB_3673738
偶联类型 CoraLite® Plus 750 Fluorescent Dye
最大激发/发射波长755 nm / 780 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP54578
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 USP14 antibody CL750-82128Download protocol
Standard Protocols
Click here to view our Standard Protocols