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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 750-conjugated USP14 Recombinant antibody

USP14 Uni-rAbTM Recombinant Antibody for WB, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

3G12

Cat no : CL750-82128

Print datasheet

Synonyms

Ubiquitin thioesterase 14, Ubiquitin carboxyl-terminal hydrolase 14, TGT, EC:3.4.19.12, Deubiquitinating enzyme 14

验证数据展示

  • WB analysis using CL750-82128

    WB analysis using CL750-82128

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-82128 (USP14 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis using CL750-82128

    WB analysis using CL750-82128

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-82128 (USP14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using CL750-82128

    FC experiment of HepG2 using CL750-82128

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated USP14 Recombinant antibody (CL750-82128, Clone:3G12)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells, C6 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 82128-1-PBS

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-82128 targets USP14 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen USP14 fusion protein Ag5979 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
Synonyms Ubiquitin thioesterase 14, Ubiquitin carboxyl-terminal hydrolase 14, TGT, EC:3.4.19.12, Deubiquitinating enzyme 14
Calculated Molecular Weight 54 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC003556
Gene Symbol USP14
Gene ID (NCBI) 9097
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP54578
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 USP14 antibody CL750-82128Download protocol
Standard Protocols
Click here to view our Standard Protocols