验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, LNCaP cells, 4T1 cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy USP15 Ready-To-Use IHC Kit. USP15 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67557-1-Ig targets USP15 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | Human, Mouse, Rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | USP15 fusion protein Ag5712 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin specific peptidase 15 |
| 别名 | Deubiquitinating enzyme 15, KIAA0529, Ubiquitin thiolesterase 15, Unph 2, UNPH4, USP15 |
| 计算分子量 | 112 kDa |
| 观测分子量 | 112 kDa |
| GenBank蛋白编号 | BC020688 |
| 基因名称 | USP15 |
| Gene ID (NCBI) | 9958 |
| RRID | AB_2882771 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9Y4E8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ubiquitin is a highly conserved eukaryotic protein that is synthesized as a fusion protein precursor, either to itself or to one of two ribosomal proteins. USP15, is also named as KIAA0529,Unph-2, Unph4, encoding a human ubiquitin-specific protease (USP). The USP15 protein contains the highly conserved Cys and His boxes present in all members of he UBP family of deubiquitinating enzymes(PMID:10444327).It has 3 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for USP15 antibody 67557-1-Ig | Download protocol |
| IHC protocol for USP15 antibody 67557-1-Ig | Download protocol |
| IF protocol for USP15 antibody 67557-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Dis The deubiquitinating enzyme USP15 stabilizes ERα and promotes breast cancer progression.
| ||
Endocr Relat Cancer LncRNA MFSD4A-AS1 promotes lymphangiogenesis and lymphatic metastasis of papillary thyroid cancer | ||
J Cancer USP15 promotes the progression of papillary thyroid cancer by regulating HMGB1 stability through its deubiquitination
| ||
Nat Commun Proteostatic reactivation of the developmental transcription factor TBX3 drives BRAF/MAPK-mediated tumorigenesis | ||
ACS Pharmacol Transl Sci Cell-Based Covalent-Capture Deubiquitinase Assay for Inhibitor Discovery | ||
Mol Med USP15 regulates radiation-induced DNA damage and intestinal injury through K48-linked deubiquitination and stabilisation of ATM |
