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验证数据展示

  • WB analysis using 67557-1-Ig (same clone as 67557-1-PBS)

    WB analysis using 67557-1-Ig (same clone as 67557-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67557-1-Ig (USP15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67557-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67557-1-Ig (same clone as 67557-1-PBS)

    IHC staining of human breast cancer using 67557-1-Ig (same clone as 67557-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67557-1-Ig (USP15 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67557-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with USP15 antibody included
  • IHC staining of human breast cancer using 67557-1-Ig (same clone as 67557-1-PBS)

    IHC staining of human breast cancer using 67557-1-Ig (same clone as 67557-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67557-1-Ig (USP15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67557-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with USP15 antibody included
  • WB analysis using 67557-1-Ig (same clone as 67557-1-PBS)

    WB analysis using 67557-1-Ig (same clone as 67557-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67557-1-Ig (USP15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67557-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

USP15 Monoclonal antibody, PBS Only

USP15 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67557-1-PBS

产品说明书

CloneNo. 1C9G3

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

WB, IHC, Indirect ELISA

别名

Deubiquitinating enzyme 15, KIAA0529, Ubiquitin thiolesterase 15, Unph 2, UNPH4, USP15

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy USP15 Ready-To-Use IHC Kit. USP15 primary antibody included.

产品信息

67557-1-PBS targets USP15 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 USP15 fusion protein Ag5712 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 15
别名 Deubiquitinating enzyme 15, KIAA0529, Ubiquitin thiolesterase 15, Unph 2, UNPH4, USP15
计算分子量 112 kDa
观测分子量 112 kDa
GenBank蛋白编号BC020688
基因名称 USP15
Gene ID (NCBI) 9958
RRIDAB_2882771
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y4E8
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ubiquitin is a highly conserved eukaryotic protein that is synthesized as a fusion protein precursor, either to itself or to one of two ribosomal proteins. USP15, is also named as KIAA0529,Unph-2, Unph4, encoding a human ubiquitin-specific protease (USP). The USP15 protein contains the highly conserved Cys and His boxes present in all members of he UBP family of deubiquitinating enzymes(PMID:10444327).It has 3 isoforms produced by alternative splicing.