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验证数据展示

  • WB analysis using 12199-1-AP (same clone as 12199-1-PBS)

    WB analysis using 12199-1-AP (same clone as 12199-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12199-1-AP (USP25 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12199-1-PBS in a different storage buffer formulation.

  • IP experiment of RAW 264.7 using 12199-1-AP (same clone as 12199-1-PBS)

    IP experiment of RAW 264.7 using 12199-1-AP (same clone as 12199-1-PBS)

    IP result of anti-USP25 (IP:12199-1-AP, 4ug; Detection:12199-1-AP 1:300) with RAW 264.7 cells lysate 1600ug. This data was developed using the same antibody clone with 12199-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 12199-1-AP (same clone as 12199-1-PBS)

    IHC staining of human lung cancer using 12199-1-AP (same clone as 12199-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12199-1-AP (USP25 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12199-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 12199-1-AP (same clone as 12199-1-PBS)

    IHC staining of human lung cancer using 12199-1-AP (same clone as 12199-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12199-1-AP (USP25 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12199-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

USP25 Polyclonal antibody, PBS Only

USP25 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12199-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

12199-1-PBS targets USP25 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 USP25 fusion protein Ag2837 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 25
别名
计算分子量 125 kDa
观测分子量 125 kDa
GenBank蛋白编号BC015930
基因名称 USP25
Gene ID (NCBI) 29761
RRIDAB_2212771
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UHP3
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ubiquitin carboxyl-terminal hydrolase 25, encoded by USP25 gene, has three isoforms by alternative splicing. Isoform USP25a is found in most adult and fetal tissues; Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific, and is upregulated during myogenesis, therefore, may have a role in the regulation of muscular differentiation and function. USP25, as deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates.