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USP36 Monoclonal antibody
USP36 Monoclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, IP, ELISA
Conjugate
Unconjugated
CloneNo.
1A1B3
验证数据展示
经过测试的应用
| Positive WB detected in | U2OS cells, K-562 cells, HeLa cells, A549 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IP detected in | Jurkat cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
68165-1-Ig targets USP36 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | USP36 fusion protein Ag6426 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | ubiquitin specific peptidase 36 |
| Synonyms | Ubiquitin carboxyl-terminal hydrolase 36, KIAA1453, EC:2.3.2.-, DUB1, Deubiquitinating enzyme 36 |
| Calculated Molecular Weight | 1123 aa, 123 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | BC038983 |
| Gene Symbol | USP36 |
| Gene ID (NCBI) | 57602 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9P275 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
USP36 (Ubiquitin carboxyl-terminal hydrolase 36) is also named as KIAA1453, FLJ12851, DUB1 and belongs to the peptidase C19 family. It regulates the transcription and processing of rRNA by deubiquitylating various proteins involved in these processes, and the aberrant nucleolar structure in its absence is secondary to deficient ribosome biogenesis. USP36 plays a crucial role in regulating the structure and function of nucleoli (PMID:19208757). The ubiquitin-specific protease that is required in germline, epithelial, and intestinal stem cells (PMID:19039105). USP36 can be detected in 150 kDa (PMID:33852194)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for USP36 antibody 68165-1-Ig | Download protocol |
| IF protocol for USP36 antibody 68165-1-Ig | Download protocol |
| IP protocol for USP36 antibody 68165-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |