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验证数据展示

  • WB analysis using 84620-3-RR (same clone as 84620-3-PBS)

    WB analysis using 84620-3-RR (same clone as 84620-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84620-3-RR (USP36 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84620-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01428-1

    Sandwich ELISA standard curve of MP01428-1

    Sandwich ELISA standard curve of MP01428-1, Human USP36 Recombinant Matched Antibody Pair - PBS only. 84620-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag6411. 84620-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • Sample test of MP01428-1

    Sample test of MP01428-1

    The mean USP36 concentration was determined to be 4,390.7 pg/mL in HeLa cell extract based on a 1.3 mg/mL extract load, 16,332.6 pg/mL in Jurkat cell extract based on a 1.5 mg/mL extract load and 3,553.0 pg/mL in HepG2 cell extract based on a 1.0 mg/mL extract load.

  • Cytometric bead array standard curve of MP01428-1

    Cytometric bead array standard curve of MP01428-1

    Cytometric bead array standard curve of MP01428-1, USP36 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84620-3-PBS. Detection antibody: 84620-1-PBS. Standard: Ag6411. Range: 0.156-20 ng/mL.

  • Cytometric bead array standard curve of MP01428-2

    Cytometric bead array standard curve of MP01428-2

    Cytometric bead array standard curve of MP01428-2, USP36 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84620-3-PBS. Detection antibody: 84620-2-PBS. Standard: Ag6411. Range: 0.156-20 ng/mL.

  • Affinity and Kinetic Characterization of 84620-3-RR

    Affinity and Kinetic Characterization of 84620-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84620-3-RR against Human USP36 were performed. The affinity constant is 3.71 nM.

USP36 Recombinant antibody, PBS Only (Capture)

USP36 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84620-3-PBS

产品说明书

CloneNo. 241962H7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

Ubiquitin carboxyl-terminal hydrolase 36, KIAA1453, EC:2.3.2.-, DUB1, Deubiquitinating enzyme 36

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84620-3-PBS targets USP36 as part of a matched antibody pair:

MP01428-1: 84620-3-PBS capture and 84620-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP01428-2: 84620-3-PBS capture and 84620-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 USP36 fusion protein Ag6411 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ubiquitin specific peptidase 36
别名 Ubiquitin carboxyl-terminal hydrolase 36, KIAA1453, EC:2.3.2.-, DUB1, Deubiquitinating enzyme 36
计算分子量 1123 aa, 123 kDa
观测分子量140 kDa
GenBank蛋白编号BC038983
基因名称 USP36
Gene ID (NCBI) 57602
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9P275
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

USP36(Ubiquitin carboxyl-terminal hydrolase 36) is also named as KIAA1453, FLJ12851, DUB1 and belongs to the peptidase C19 family. It regulates the transcription and processing of rRNA by deubiquitylating various proteins involved in these processes, and the aberrant nucleolar structure in its absence is secondary to deficient ribosome biogenesis. USP36 plays a crucial role in regulating the structure and function of nucleoli.(PMID:19208757). The ubiquitin-specific protease that is required in germline, epithelial, and intestinal stem cells(PMID:19039105). It has 2 isoforms produced by alternative splicing.