验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, JurKat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84620-3-RR targets USP36 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | USP36 fusion protein Ag6411 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ubiquitin specific peptidase 36 |
| 别名 | Ubiquitin carboxyl-terminal hydrolase 36, KIAA1453, EC:2.3.2.-, DUB1, Deubiquitinating enzyme 36 |
| 计算分子量 | 1123 aa, 123 kDa |
| 观测分子量 | 140 kDa |
| GenBank蛋白编号 | BC038983 |
| 基因名称 | USP36 |
| Gene ID (NCBI) | 57602 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9P275 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
USP36(Ubiquitin carboxyl-terminal hydrolase 36) is also named as KIAA1453, FLJ12851, DUB1 and belongs to the peptidase C19 family. It regulates the transcription and processing of rRNA by deubiquitylating various proteins involved in these processes, and the aberrant nucleolar structure in its absence is secondary to deficient ribosome biogenesis. USP36 plays a crucial role in regulating the structure and function of nucleoli.(PMID:19208757). The ubiquitin-specific protease that is required in germline, epithelial, and intestinal stem cells(PMID:19039105). It has 2 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for USP36 antibody 84620-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |