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USP4 Monoclonal antibody, PBS Only

USP4 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1G11G8

Cat no : 66822-1-PBS

Print datasheet

Synonyms

Unph, UNP, Ubiquitin-specific-processing protease 4, Ubiquitin carboxyl-terminal hydrolase 4, EC:3.4.19.12

验证数据展示

  • WB analysis using 66822-1-Ig (same clone as 66822-1-PBS)

    WB analysis using 66822-1-Ig (same clone as 66822-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66822-1-Ig (USP4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66822-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66822-1-Ig (same clone as 66822-1-PBS)

    IHC staining of human breast cancer using 66822-1-Ig (same clone as 66822-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66822-1-Ig (USP4 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66822-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66822-1-Ig (same clone as 66822-1-PBS)

    IHC staining of human breast cancer using 66822-1-Ig (same clone as 66822-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66822-1-Ig (USP4 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66822-1-PBS in a different storage buffer formulation.

  • FC experiment of THP-1 using 66822-1-Ig (same clone as 66822-1-PBS)

    FC experiment of THP-1 using 66822-1-Ig (same clone as 66822-1-PBS)

    1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human USP4 (66822-1-Ig, Clone:1G11G8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66822-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 66822-1-Ig (same clone as 66822-1-PBS)

    IF Staining of human breast cancer using 66822-1-Ig (same clone as 66822-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using USP4 antibody (66822-1-Ig, Clone: 1G11G8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66822-1-PBS in a different storage buffer formulation.

产品信息

66822-1-PBS targets USP4 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IHC, IF-P, FC (Intra), ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen USP4 fusion protein Ag28297 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name ubiquitin specific peptidase 4 (proto-oncogene)
Synonyms Unph, UNP, Ubiquitin-specific-processing protease 4, Ubiquitin carboxyl-terminal hydrolase 4, EC:3.4.19.12
Calculated Molecular Weight 963 aa, 109 kDa
Observed Molecular Weight 109 kDa
GenBank Accession NumberBC125131
Gene Symbol USP4
Gene ID (NCBI) 7375
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13107
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

USP4, also named as UNP and UNPH, belongs to the peptidase C19 family and USP4 subfamily. USP4 is a Deubiquitinating enzymes that links to mitogen-activated protein kinase signaling, pre-mRNA splicing and control of p53 stability (PMID: 26455393). USP4 has 3 isoforms with the molecular mass of 36, 104 and 109 kDa. Recently, it has been reported that USP4 is a critical factor in promoting lung cancer stemness and potentially useful lung cancer prognosis marker (PMID: 32549341).