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验证数据展示

  • IF Staining of A549 using CL488-66213

    IF Staining of A549 using CL488-66213

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 USP5 antibody (CL488-66213, Clone: 3F12C10 ) at dilution of 1:400.

  • FC experiment of A549 using CL488-66213

    FC experiment of A549 using CL488-66213

    1x10^6 A549 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated USP5 Monoclonal antibody (CL488-66213, Clone:3F12C10)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated USP5 Monoclonal antibody

USP5 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66213

产品说明书

CloneNo. 3F12C10

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

ISOT 1, ISOT, Isopeptidase T, EC:3.4.19.12, Deubiquitinating enzyme 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA549 cells
For other applications, we recommend the unconjugated version of this antibody, 66213-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66213 targets USP5 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 USP5 fusion protein Ag7575 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 5 (isopeptidase T)
别名 ISOT 1, ISOT, Isopeptidase T, EC:3.4.19.12, Deubiquitinating enzyme 5
计算分子量 96 kDa
观测分子量 95-105 kDa
GenBank蛋白编号BC005139
基因名称 USP5
Gene ID (NCBI) 8078
RRIDAB_2934452
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP45974
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP5, also named as ISOT, belongs to the peptidase C19 family. Knock-down of USP5 causes the accumulation of TP53/p53 and an increase in TP53/p53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated TP53/p53 but not with MDM2 for proteasomal recognition. USP5 is a potential target for p53 activating therapeutic agents for the treatment of cancer(PMID: 19098288). It is a novel proteasome associated protein(PMID: 19182904). USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 USP5 antibody CL488-66213Download protocol
FC protocol for CL Plus 488 USP5 antibody CL488-66213Download protocol
Standard Protocols
Click here to view our Standard Protocols