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CoraLite® Plus 488-conjugated USP5 Monoclonal antibody
USP5 Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3F12C10
验证数据展示
经过测试的应用
Positive IF/ICC detected in | A549 cells |
Positive FC (Intra) detected in | A549 cells |
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-66213 targets USP5 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, FC (Intra) Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | USP5 fusion protein Ag7575 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | ubiquitin specific peptidase 5 (isopeptidase T) |
Synonyms | ISOT 1, ISOT, Isopeptidase T, EC:3.4.19.12, Deubiquitinating enzyme 5 |
Calculated Molecular Weight | 96 kDa |
Observed Molecular Weight | 95-105 kDa |
GenBank Accession Number | BC005139 |
Gene Symbol | USP5 |
Gene ID (NCBI) | 8078 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P45974 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 USP5 antibody CL488-66213 | Download protocol |
FC protocol for CL Plus 488 USP5 antibody CL488-66213 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |