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验证数据展示

  • IF Staining of HeLa using CL488-66514

    IF Staining of HeLa using CL488-66514

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 USP7 antibody (CL488-66514, Clone: 3D7D11 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-66514

    FC experiment of HeLa using CL488-66514

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human USP7 (CL488-66514, Clone:3D7D11) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated USP7 Monoclonal antibody

USP7 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66514

产品说明书

CloneNo. 3D7D11

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

HAUSP, 3D7D11

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy USP7 Ready-To-Use IHC Kit. USP7 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66514-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66514 targets USP7 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 USP7 fusion protein Ag25652 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 7 (herpes virus-associated)
别名 HAUSP, 3D7D11
观测分子量 128 kDa
基因名称 USP7
Gene ID (NCBI) 7874
RRIDAB_2934472
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ93009
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 USP7 antibody CL488-66514Download protocol
FC protocol for CL Plus 488 USP7 antibody CL488-66514Download protocol
Standard Protocols
Click here to view our Standard Protocols