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CoraLite® Plus 488-conjugated USP8 Monoclonal antibody

USP8 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E12E3

Cat No : CL488-67321

Print datasheet

Synonyms

Ubiquitin carboxyl-terminal hydrolase 8, KIAA0055, HumORF8, hUBPy, EC:3.4.19.12

验证数据展示

  • IF Staining of HeLa using CL488-67321

    IF Staining of HeLa using CL488-67321

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 USP8 antibody (CL488-67321, Clone: 1E12E3 ) at dilution of 1:200.

  • FC experiment of Hela using CL488-67321

    FC experiment of Hela using CL488-67321

    1X10^6 Hela cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human USP8 (CL488-67321, Clone:1E12E3) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHela cells
For other applications, we recommend the unconjugated version of this antibody, 67321-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67321 targets USP8 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen USP8 fusion protein Ag27104 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ubiquitin specific peptidase 8
Synonyms Ubiquitin carboxyl-terminal hydrolase 8, KIAA0055, HumORF8, hUBPy, EC:3.4.19.12
Calculated Molecular Weight 1118 aa, 128 kDa
Observed Molecular Weight 128 kDa
GenBank Accession NumberBC110590
Gene Symbol USP8
Gene ID (NCBI) 9101
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP40818
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 USP8 antibody CL488-67321Download protocol
FC protocol for CL Plus 488 USP8 antibody CL488-67321Download protocol
Standard Protocols
Click here to view our Standard Protocols