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验证数据展示

  • IF Staining of HeLa using CL594-55054

    IF Staining of HeLa using CL594-55054

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 USP9X antibody (CL594-55054) at dilution of 1:200.

  • FC experiment of HeLa using CL594-55054

    FC experiment of HeLa using CL594-55054

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human USP9X (CL594-55054) (red), or 0.8 ug CoraLite®594-conjugated Rabbit IgG control Rabbit PolyAb (CL594-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated USP9X Polyclonal antibody

USP9X Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-55054

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

Fat facets protein-related, X-linked, FAM, FAF, EC:3.4.19.12, DFFRX

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 55054-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-55054 targets USP9X in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 9, X-linked
别名 Fat facets protein-related, X-linked, FAM, FAF, EC:3.4.19.12, DFFRX
计算分子量 292 kDa
观测分子量 260-290 kDa
GenBank蛋白编号NM_001039590
基因名称 USP9X
Gene ID (NCBI) 8239
RRIDAB_2919884
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ93008
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP9X, also named as DFFRX, FAM and USP9, belongs to the peptidase C19 family. It may function as a ubiquitin-protein or polyubiquitin hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. It plays an important role regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. USP9X is an essential component of TGF-beta/BMP signaling cascade. Deubiqitination of SMAD4 by USP9X restores its competence to mediate TGF-beta signaling. USP9X regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. The antibody is specific to USP9X. It has no cross reaction to USP9Y.

实验方案

Product Specific Protocols
IF protocol for CL594 USP9X antibody CL594-55054Download protocol
Standard Protocols
Click here to view our Standard Protocols